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Concentration Dependent Approach for Accurate Determination of Two-Photon Absorption Cross-Section of Fluorescent dye Molecule

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Abstract

We have investigated the concentration dependent of two-photon induced fluorescence (TPIF) in methanolic solution of Rhodamine 6G and Rhodamine B dye using 120 fs laser pulses at 780 nm, 76 MHz repetition rate. TPIF study of these dyes was compared with their respective one photon fluorescence intensity. We have shown the effect of chopper on TPIF intensity from Rhodamine dyes, which have shown direct influence on the determined TPA Cross section of these dyes.

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References

  1. Goeppert-Mayer M (1931) Fiber Elementarakte mit xwei QuantemspWwgem. Ann Phys 9:273–295

    Article  Google Scholar 

  2. He GS, Tan L-S, Zheng Q, Prasad PN (2008) Multiphoton absorbing materials: molecular designs, characterizations, and applications. Chem Rev 108:1245–1330

    Article  CAS  PubMed  Google Scholar 

  3. Albota M, Xu C, Webb WW (1998) Two-photon fluorescence excitation cross sections of biomolecular probes from 690 to 960 nm. Appl Opt 37:7352–7356

    Article  CAS  PubMed  Google Scholar 

  4. Chen Z, Kaplan DL, Yang K, Kumar J, Marx KA, Tripathy SK (1997) Two-photon-induced fluorescence from the phycoerythrin protein. Appl Opt 36:1655–1659

    Article  CAS  PubMed  Google Scholar 

  5. Meshalkin YP, Svetlichnyi VA, Chunosova SS, Kopylova TN (2005) Two-photon induced fluorescence and electronic structure of substituted dicyanomethylene pyrans in solutions under femtosecond excitation. Russ Phys J 48:1182–1187

    Article  CAS  Google Scholar 

  6. Mathai S, Bird DK, Stylli SS, Smith TA, Ghigginoa KP (2007) Two-photon absorption cross-sections and time-resolved fluorescence imaging using porphyrin photosensitisers. Photochem Photobiol Sci 6:1019–1026

    Article  CAS  PubMed  Google Scholar 

  7. Jha PC, Wang Y, Agren H (2008) Two-photon absorption cross-sections of reference dyes: a critical examination. Chem Phys Chem 9:111–116

    Article  CAS  Google Scholar 

  8. Singha S, Kim D, Rao AS, Wang T, Kim KH, Lee K-H, Kim K-T, Ahn KH (2013) Two-photon probes based on arylsulfonyl azides: fluorescence detection and imaging of biothiols. Dyes Pigments 99:308–315

    Article  CAS  Google Scholar 

  9. A. Nag, A. K. De, D. Goswami (2009). Two-photon cross-section measurements using an optical chopper: z-scan and two-photon fluorescence schemes. J Phys B Atomic Mol Phys 42: 065103. (1–9).

  10. Luchowski R (2011) Two-photon excitation of 2,5-diphenyloxazole using a low power green solid state laser. Chem Phys Lett 501:572–574

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Penzkofer A, Leupacher W (1987) Fluorescence behavior of highly concentrated rhodamine 6G solutions. J Lumin 37:61–72

    Article  CAS  Google Scholar 

  12. Faraggi M, Peretz P, Rosenthal I, Weinraub D (1984) Solution properties of dye lasers. Rhodamine B Alcohols Chem Phys Lett 103:310–314

    Article  CAS  Google Scholar 

  13. Markarov NS, Drobizhev M, Rebane A (2008) Two-photon absorption standards in the 550–1600 nm excitation wavelength range. Opt Express 16:4029–4047

    Article  Google Scholar 

  14. Rummy M, Perry JW (2010) Two-photon absorption: an overview of measurements and principles. Adv Opt Photon 2:451–518

    Article  Google Scholar 

  15. Diaspro A, Chirico G, Collini M (2005) Two-photon fluorescence excitation and related techniques in biological microscopy. Q Rev Biophys 38:97–166

    Article  CAS  PubMed  Google Scholar 

  16. Xu C, Webb WW (1996) Measurement of two-photon excitation cross sections of molecular fluorophores with data from 690 to 1050 nm. J Opt Soc Am B 13:481–491

    Article  CAS  Google Scholar 

  17. De AK, Goswami D (2009) Exploring the nature of photo-damage in two-photon excitation by fluorescence intensity modulation. J Fluoresc 19:381–386

    Article  CAS  PubMed  Google Scholar 

  18. Tian P, Warren WS (2002) Ultrafast measurement of two-photon absorption by loss modulation. Opt Lett 27:1634–1636

    Article  PubMed  Google Scholar 

  19. Fischer MC, Liu HC, Piletic IR, Warren WS (2008) Simultaneous self-phase modulation and two-photon absorption measurement by a spectral homodyne Z-scan method. Opt Express 16:4192–4205

    Article  PubMed  Google Scholar 

  20. Lu Y, Penzkofer A (1986) Absorption behavior of methanolic rhodamine 6G solutions at high concentration. Chem Phys 107:175–184

    Article  CAS  Google Scholar 

  21. Selanger KA, Falnes J, Sikkeland T (1977) Fluorescence lifetime studies of rhodamine 6G in methanol. J Phys Chem 81:1960–1963

    Article  CAS  Google Scholar 

  22. Bindhu CV, Harilal SS, Varier GK, Issac RC, Nampoori VPN, Vallabhan CPG (1996) Measurement of the absolute fluorescence quantum yield of rhodamine B solution using a dual-beam thermal lens technique. J Phys D Appl Phys 29:1074–1079

    Article  CAS  Google Scholar 

  23. Bindhu CV, Harilal SS (2001) Effect of the excitation source on the quantum-yield measurement of rhodamine B laser dye studied using thermal-lens technique. Anal Sci 17:141–144

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Chemistry, Indian Institute of Technology, Kanpur, 208016, India

    Sandeep Kumar Maurya, Chayan Dutta & Debabrata Goswami

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  1. Sandeep Kumar Maurya
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  2. Chayan Dutta
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  3. Debabrata Goswami
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Correspondence to Debabrata Goswami.

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Maurya, S.K., Dutta, C. & Goswami, D. Concentration Dependent Approach for Accurate Determination of Two-Photon Absorption Cross-Section of Fluorescent dye Molecule. J Fluoresc 27, 1399–1403 (2017). https://doi.org/10.1007/s10895-017-2076-4

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  • DOI: https://doi.org/10.1007/s10895-017-2076-4

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