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Studying the Interaction of Pirarubicin with DNA and Determining Pirarubicin in Human Urine Samples: Combining Excitation -Emission Fluorescence Matrices with Second-order Calibration Methods

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Abstract

In this paper, UV–vis spectroscopy and fluorescence were combined to study the binding of Calf thymus DNA (ct-DNA) with the anthacycline antibiotic drug pirarubicin (THP). Ethidium bromide (EB) as the fluorescence probe was used to study the competitive binding interactions of THP with DNA by excitation -emission fluorescence matrices (EEFMs) coupled with the parallel factor analysis (PARAFAC) and the alternating normalization-weighted error algorithm (ANWE) with the second-order advantage. All the results conformed that THP mainly bound with DNA by intercalation. Meanwhile, the two second-order calibration methods have been successfully applied to quantify THP in urine samples. Figures of merit were applied to compare the performance of the two methods. The results presented in this work showed that both the PARAFAC and ANWE methods were the convincing way to be applied in the complex biological systems even in the presence of uncalibrated interferences.

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Acknowledgements

The authors would like to thank the National Nature Science Foundation of China (Grant Nos. 20775025 and 20435010) and the National 973 Project (2007CB216404) for financial supports.

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  1. State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P. R. China

    Hong-Yan Zou, Hai-Long Wu, Yan Zhang, Shu-Fang Li, Jin-Fang Nie, Hai-Yan Fu & Ru-Qin Yu

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  1. Hong-Yan Zou
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Zou, HY., Wu, HL., Zhang, Y. et al. Studying the Interaction of Pirarubicin with DNA and Determining Pirarubicin in Human Urine Samples: Combining Excitation -Emission Fluorescence Matrices with Second-order Calibration Methods. J Fluoresc 19, 955–966 (2009). https://doi.org/10.1007/s10895-009-0495-6

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