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Properties of Mixed Cationic Membranes studied by Fluorescence Solvent Relaxation

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Abstract

The need for detailed biophysical description of cationic lipid membranes, which are commonly used as gene transfection vectors, led us to study the properties of mixed cationic/zwitterionic lipid bilayers. Fluorescence solvent relaxation measurements of 6-dodecanoyl-2-dimethylaminonaphthalene (Laurdan) incorporated in a membrane consisting of cationic dimyristoyltrimethylammoniumpropane (DMTAP) and zwitterionic dimyristoylphosphatidylcholine (DMPC) were performed. The obtained results are compared with a recently measured system consisting of dioleoyltrimethylammoniumpropane (DOTAP) and dioleoylphosphatidylcholine (DOPC) (Jurkiewicz et al. Langmuir 22:8741–8749, 2006). The similar nonmonotonic dependence of the relaxation kinetics on cationic lipid content in the membrane was present for both systems. While the slowest solvent relaxation have been observed for 30 mol% of DOTAP in the DOPC bilayer (Jurkiewicz et al. Langmuir 22:8741–8749, 2006), for DMPC/DMTAP system it was found at 45 mol% of DMTAP, which agrees with the literature. Both membranes increased their hydration upon increased cationic lipid content.

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References

  1. Jurkiewicz P, Olzynska A, Langner M, Hof M (2006) Headgroup hydration and mobility of DOTAP/DOPC bilayers: a fluorescence solvent relaxation study. Langmuir 22:8741–8749

    Article  PubMed  CAS  Google Scholar 

  2. Felgner PL, Gadek TR, Holm M, Roman R, Chan HW, Wenz M, Northrop JP, Ringold GM, Danielsen M (1987) Lipofection—a highly efficient, lipid-mediated DNA-transfection procedure. Proc Natl Acad Sci U S A 84(21):7413–7417

    Article  PubMed  CAS  Google Scholar 

  3. Kral T, Langner M, Benes M, Baczynska D, Ugorski M, Hof M (2002) The application of fluorescence correlation spectroscopy in detecting DNA condensation. Biophys Chem 95(2):135–144

    Article  PubMed  CAS  Google Scholar 

  4. Regelin AE, Fankhaenel S, Gurtesch L, Prinz C, von-Kiedrowski G, Massing U (2000) Biophysical and lipofection studies of DOTAP analogs. Biochim Biophys Acta 1464:151–164

    Article  PubMed  CAS  Google Scholar 

  5. Hui SW, Langner M, Zhao YL, Ross P, Hurley E, Chan K (1996) The role of helper lipids in cationic liposome-mediated gene transfer. Biophys J 71(2):590–599

    Article  PubMed  CAS  Google Scholar 

  6. Silvius JR (1991) Anomalous mixing of zwitterionic and anionic phospholipids with double-chain cationic amphiphiles in lipid bilayers. Biochim Biophys Acta 1070(1):51–59

    Article  PubMed  CAS  Google Scholar 

  7. Scherer PG, Seelig J (1989) Electric charge effects on phospholipid headgroups—phosphatidylcholine in mixtures with cationic and anionic amphiphiles. Biochemistry 28(19):7720–7728

    Article  PubMed  CAS  Google Scholar 

  8. Roux M, Neumann JM, Hodges RS, Devaux PF, Bloom M (1989) Conformational-changes of phospholipid headgroups induced by a cationic integral membrane peptide as seen by deuterium magnetic-resonance. Biochemistry 28(5):2313–2321

    Article  PubMed  CAS  Google Scholar 

  9. Jurkiewicz P, Sykora J, Olzynska A, Humplickova J, Hof M (2005) Solvent relaxation in phospholipid bilayers: principles and recent applications. J Fluoresc 15(6):883–894

    Article  PubMed  CAS  Google Scholar 

  10. Sykora J, Hof M (2002) Solvent relaxation in phospholipid bilayers: physical understanding and biophysical applications. Cell Mol Biol Lett 7(2):259–261

    PubMed  Google Scholar 

  11. Sykora J, Kapusta P, Fidler V, Hof M (2002) On what time scale does solvent relaxation in phospholipid bilayers happen? Langmuir 18(3):571–574

    Article  CAS  Google Scholar 

  12. Gurtovenko AA, Patra M, Karttunen M, Vattulainen I (2004) Cationic DMPC/DMTAP lipid bilayers: molecular dynamics study. Biophys J 86(6):3461–3472

    Article  PubMed  CAS  Google Scholar 

  13. Levadny V, Yamazaki M (2005) Cationic DMPC/DMTAP lipid bilayers: local lateral polarization of phosphatidylcholine headgroups. Langmuir 21(13):5677–5680

    Article  PubMed  CAS  Google Scholar 

  14. Hutterer R, Parusel ABJ, Hof M (1998) Solvent relaxation of Prodan and Patman: a useful tool for the determination of polarity and rigidity changes in membranes. J Fluoresc 8(4):389–393

    Article  CAS  Google Scholar 

  15. Olzynska A, Zan A, Jurkiewicz P, Sykora J, Grobner G, Langner M, Hof M (2007) Molecular interpretation of fluorescence solvent relaxation of Patman and 2H NMR experiments in phosphatidylcholine bilayers. Chem Phys Lipids 147:69–77

    Article  PubMed  CAS  Google Scholar 

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Acknowledgements

Financial support by the Grant Agency of the Academy of Sciences of the Czech Republic (P. J., A. O. via A400400503) and the Ministry of Education and Sport of the Czech Republic (M.H. via LC06063) is gratefully acknowledged.

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Authors and Affiliations

  1. J. Heyrovský Institute of Physical Chemistry of the ASCR, v. v. i., Dolejškova 3, Prague 8, 18223, Czech Republic

    Agnieszka Olżyńska, Piotr Jurkiewicz & Martin Hof

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  1. Agnieszka Olżyńska
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  2. Piotr Jurkiewicz
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  3. Martin Hof
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Correspondence to Martin Hof.

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Olżyńska, A., Jurkiewicz, P. & Hof, M. Properties of Mixed Cationic Membranes studied by Fluorescence Solvent Relaxation. J Fluoresc 18, 925–928 (2008). https://doi.org/10.1007/s10895-008-0321-6

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  • DOI: https://doi.org/10.1007/s10895-008-0321-6

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