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Fluorescence Lifetime Correlation Spectroscopy

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Abstract

This article explains the basic principles of FLCS, a genuine fusion of Time-Correlated Single Photon Counting (TCSPC) and Fluorescence Correlation Spectroscopy (FCS), using common terms and minimum mathematics. The usefulness of the method is demonstrated on simple FCS experiments. The method makes possible to separate the autocorrelation function of individual components of a mixture of fluorophores, as well as purging the result from parasitic contributions like scattered light or detector afterpulsing.

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Acknowledgment

A.B. and M.H. acknowledge financial support by the Ministry of Education and Sport of the Czech Republic via grant LC06063.

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Authors and Affiliations

  1. PicoQuant GmbH, Rudower Chaussee 29, 12489, Berlin, Germany

    Peter Kapusta & Michael Wahl

  2. J. Heyrovský Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, Dolejškova 3, 18223, Prague, Czech Republic

    Aleš Benda & Martin Hof

  3. IBI-1, Forschungszentrum Jülich in der Helmholtzgesellschaft, 52425, Jülich, Germany

    Jörg Enderlein

Authors
  1. Peter Kapusta
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  2. Michael Wahl
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  3. Aleš Benda
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  4. Martin Hof
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  5. Jörg Enderlein
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Corresponding author

Correspondence to Peter Kapusta.

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Kapusta, P., Wahl, M., Benda, A. et al. Fluorescence Lifetime Correlation Spectroscopy. J Fluoresc 17, 43–48 (2007). https://doi.org/10.1007/s10895-006-0145-1

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  • DOI: https://doi.org/10.1007/s10895-006-0145-1

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