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Multi-receiver solid-state NMR using polarization optimized experiments (POE) at ultrafast magic angle spinning

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Abstract

Ultrafast magic angle spinning (MAS) technology and 1H detection have dramatically enhanced the sensitivity of solid-state NMR (ssNMR) spectroscopy of biopolymers. We previously showed that, when combined with polarization optimized experiments (POE), these advancements enable the simultaneous acquisition of multi-dimensional 1H- or 13C-detected experiments using a single receiver. Here, we propose a new sub-class within the POE family, namely HC-DUMAS, HC-MEIOSIS, and HC-MAeSTOSO, that utilize dual receiver technology for the simultaneous detection of 1H and 13C nuclei. We also expand this approach to record 1H-, 13C-, and 15N-detected homonuclear 2D spectra simultaneously using three independent receivers. The combination of POE and multi-receiver technology will further shorten the total experimental time of ssNMR experiments for biological solids.

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Acknowledgements

This work was supported by the National Institute of Health (GM 64742, HL 144130, 1S10OD021536 to G.V.), the American Heart Association (19POST34420009 to D.W.), and the Minnesota NMR Center. We also thank Dr. J. Struppe and Dr. S. Pawsey from Bruker R&D for helpful discussions.

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  1. Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, 6-155 Jackson Hall, Minneapolis, MN, 55455, USA

    T. Gopinath, Daniel K. Weber & Gianluigi Veglia

  2. Department of Chemistry, University of Minnesota, Minneapolis, MN, 55455, USA

    Gianluigi Veglia

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  1. T. Gopinath
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Correspondence to Gianluigi Veglia.

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Gopinath, T., Weber, D.K. & Veglia, G. Multi-receiver solid-state NMR using polarization optimized experiments (POE) at ultrafast magic angle spinning. J Biomol NMR 74, 267–285 (2020). https://doi.org/10.1007/s10858-020-00316-y

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