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Rhodamine-loaded poly(lactic-co-glycolic acid) nanoparticles for investigation of in vitro interactions with breast cancer cells

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Abstract

Nanoparticle-based drug delivery systems are considered promising for the delivery of imaging agents and drugs for the detection and treatment of illnesses, including cancer. Investigation of nanoparticle interactions with the diseased cells can lead to better designs. In this work, poly(lactic-co-glycolic acid) nanoparticles loaded with rhodamine 6G were prepared by nanoprecipitation with high encapsulation efficiency. In vitro release studies demonstrated that rhodamine escaped from the nanoparticles at a very slow rate at physiological pH, thus making it ideal for imaging studies. At acidic pH this agent was released quickly, suggesting charge interactions between the polymer and rhodamine. Microscopy and flow cytometry studies show higher uptake in MDA-MB-231 breast cancer cells when exposed to rhodamine-loaded nanoparticles than to rhodamine in solution.

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Acknowledgements

This work was supported in part by the National Science Foundation Integrative Graduate Education and Research Traineeship fellowship to T. Betancourt and The University of Texas at Austin Undergraduate Research Fellowship to K. Shah. We would like to thank the Microscopy and Imaging Facility of the Institute for Cellular and Molecular Biology at The University of Texas at Austin for access to microscopy and flow cytometry equipment.

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Authors and Affiliations

  1. Department of Biomedical Engineering, The University of Texas at Austin, 1 University Station, Austin, TX, 78712, USA

    Tania Betancourt, Kunal Shah & Lisa Brannon-Peppas

  2. Appian Labs, LLC, 11412 Bee Caves Road, Suite 300, Austin, TX, 78738, USA

    Lisa Brannon-Peppas

Authors
  1. Tania Betancourt
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  2. Kunal Shah
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  3. Lisa Brannon-Peppas
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Correspondence to Lisa Brannon-Peppas.

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Betancourt, T., Shah, K. & Brannon-Peppas, L. Rhodamine-loaded poly(lactic-co-glycolic acid) nanoparticles for investigation of in vitro interactions with breast cancer cells. J Mater Sci: Mater Med 20, 387–395 (2009). https://doi.org/10.1007/s10856-008-3594-z

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  • DOI: https://doi.org/10.1007/s10856-008-3594-z

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