Abstract
The phosphorylated glycoprotein osteopontin (OPN) is involved in the regulation of biomineralization under normal and pathological conditions. Its actions include inhibiting apatite crystal growth and promoting the formation and function of mineral resorbing cells, including osteoclasts (OCL). The purpose of this study was to develop stable apatitic mineral surfaces and determine their influence on OCL formation and mineral resorption from bone marrow macrophages derived from OPN wild-type (OPN+/+) and OPN deficient (OPN−/−) mice. We demonstrated that these mineral coatings were stable and supported bone marrow-derived macrophage differentiation to OCL under our culture conditions. Macrophages harvested from OPN−/− mice had a greater capacity to form OCL than macrophages from OPN+/+ mice when allowed to differentiate on tissue culture plastic. In contrast, when allowed to differentiate on a mineral surface, no difference in OCL formation was observed. Interestingly, OPN+/+ OCL were more efficient at mineral dissolution than OPN−/− OCL, and this difference was observed regardless of differentiating surface. Our results suggest that mineralized substrates as well as ability to synthesize OPN both control OCL function in our model system. The exact nature of these effects may be dependent on variables related to mineral substrate presentation.
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Acknowledgments
This work was supported by NIH HL62329 and NIH training grant HL07828-06. The authors would like to thank Lara Gamble for performing XPS analysis on the mineral plates at NESAC/BIO, supported by NIBIB grant EB-002027; and the lab of Dr. Stephen M. Schwartz at the University of Washington for protocols regarding macrophage isolation and L929 fibroblast cells required for preparation of supplemented media. Electron microscopy work was performed at the University of Washington Nanotech User Facility (NTUF), a member of the National Nanotechnology Infrastructure Network (NNIN), which is supported by the National Science Foundation.
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Rajachar, R.M., Truong, A.Q. & Giachelli, C.M. The influence of surface mineral and osteopontin on the formation and function of murine bone marrow-derived osteoclasts. J Mater Sci: Mater Med 19, 3279–3285 (2008). https://doi.org/10.1007/s10856-008-3455-9
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DOI: https://doi.org/10.1007/s10856-008-3455-9