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A major QTL associated with cold tolerance at seedling stage in rice (Oryza sativa L.)

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Abstract

Chilling injury is one of the most important limiting factors affecting rice production in temperate and high-elevation areas. In this study, 146 microsatellite markers were employed to identify quantitative trait loci (QTL) conferring cold tolerance at seedling stage (CTS) .The mapping population consisted of 193 doubled haploid (DH) lines, which derived from a cross between a cold-tolerant japonica variety (AAV002863) and a cold-sensitive indica cultivar (Zhenshan97B). Tolerance to cold was assessed by the survival percentage of seedlings after cold treatment. In a climate chamber, after treatment at 6°C/10°C for 7 d, the measurement was taken on the sixth day of the recovery stage at room temperature. The phenotypic distribution of the DH population approximately fitted normality with skewness and kurtosis less than 0.3, and the difference among the three repetitions was not significant. Five main effect QTLs were identified with LOD > 4.0 on chromosomes 1, 2, 8 using a composite interval mapping approach. The accumulated contribution of the five QTLs was 62.28%, and a major QTL (LOD = 15.09) was identified on chromosome 2 flanked by RM561 and RM341, which explained 27.42% of the total phenotypic variation. Four significant epistatic interactions were also detected with a total contribution of 20.14%.

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Acknowledgements

This work was supported by the Program of Shanghai Qiming Star (04qmx1458) and the 973 program (2004CB117204).

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Authors and Affiliations

  1. Shanghai Agrobiological Gene Center, Shanghai, 201106, China

    Qiaojun Lou, Liang Chen, Jun Li, Xiaoyan Xu, Hanwei Mei & Lijun Luo

  2. Huazhong Agriculture University, Wuhan, 430070, China

    Yongzhong Xing & Lijun Luo

  3. China National Rice Institute, Hangzhou, 310006, China

    Zongxiu Sun

Authors
  1. Qiaojun Lou
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  2. Liang Chen
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  3. Zongxiu Sun
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  4. Yongzhong Xing
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  5. Jun Li
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  6. Xiaoyan Xu
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  7. Hanwei Mei
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  8. Lijun Luo
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Corresponding author

Correspondence to Lijun Luo.

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Liang Chen and Qiaojun Lou had made the equal contribution for the research.

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Lou, Q., Chen, L., Sun, Z. et al. A major QTL associated with cold tolerance at seedling stage in rice (Oryza sativa L.). Euphytica 158, 87–94 (2007). https://doi.org/10.1007/s10681-007-9431-5

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  • DOI: https://doi.org/10.1007/s10681-007-9431-5

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