Abstract
We report the isolation and characterization of 11 polymorphic tetranucleotide microsatellite loci in the common genet (Genetta genetta) from genomic libraries enriched for (AAAG) n and (AGAT) n repeat sequences. We chose to develop tetranucleotide repeats because they can be scored less ambiguously. In a sample of 25 individuals, we observed between four and thirteen alleles per locus and their observed and expected heterozygosities ranged from 0.60 to 0.84 and from 0.68 to 0.92, respectively. All genotypic frequencies conformed to Hardy–Weinberg equilibrium expectations and there were no instances of linkage disequilibrium detected between pairs of loci. These loci will be of use in studies of population genetics, historical demography, and molecular ecology of the common genet.
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Acknowledgments
We thank BRISA for their invaluable contributions to the collection of Portuguese carnivore samples maintained at the CBA. We also thank Mohsen Kalboussi, Faouzia Charfi-Cheikrouah, and the Direction Générale des Forêts, Tunis, for their help in obtaining common genet samples from Tunisia. We acknowledge financial support from the FCT (MCTES, Portugal), through the research projects POCI/BIA-BDE/61491/2004 (POCI 2010 program) and PPCDT/BIA-BDE/61491/2004, and through the postdoctoral fellowship awarded to Carlos Fernandes (BPD/20317/2004).
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Carlos A. Fernandes and Mónica Rodrigues contributed equally to this work.
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Fernandes, C.A., Rodrigues, M., Hajji, G.M. et al. Isolation and characterisation of 11 tetranucleotide microsatellite loci in the common genet (Genetta genetta). Conserv Genet 10, 1931 (2009). https://doi.org/10.1007/s10592-009-9858-4
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DOI: https://doi.org/10.1007/s10592-009-9858-4