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Molecular and cytogenetic characterization of a durum wheat–Aegilops speltoides chromosome translocation conferring resistance to stem rust

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Abstract

Stem rust is a serious disease of wheat that has caused historical epidemics, but it has not been a threat in recent decades in North America owing to the eradication of the alternative host and deployment of resistant cultivars. However, the recent emergence of Ug99 (or race TTKS) poses a threat to global wheat production because most currently grown wheat varieties are susceptible. In this study, we evaluated a durum wheat–Aegilops speltoides chromosome translocation line (DAS15) for reaction to Ug99 and six other races of stem rust, and used molecular and cytogenetic tools to characterize the translocation. DAS15 was resistant to all seven races of stem rust. Two durum–Ae. speltoides translocated chromosomes were detected in DAS15. One translocation involved the short arm, centromere, and a major portion of the long arm of Ae. speltoides chromosome 2S and a small terminal segment from durum chromosome arm 2BL. Thus, this translocated chromosome is designated T2BL-2SL•2SS. Cytogenetic mapping assigned the resistance gene(s) in DAS15 to the Ae. speltoides segment in T2BL-2SL•2SS. The Ae. speltoides segment in the other translocated chromosome did not harbour stem rust resistance. A comparison of DAS15 and the wheat stocks carrying the Ae. speltoides-derived resistance genes Sr32 and Sr39 indicated that stem rust resistance gene present in DAS15 is likely novel and will be useful for developing germplasm with resistance to Ug99. Efforts to reduce Ae. speltoides chromatin in T2BL-2SL•2SS are currently in progress.

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Abbreviations

CS:

Chinese Spring wheat

FGISH:

fluorescence genomic in-situ hybridization

FITC:

fluorescein isothiocyanate

IT:

infection type

LDN:

Langdon durum

NT:

nullisomic-tetrasomic

PCR:

polymerase chain reaction

RFLP:

restriction fragment length polymorphism

SSR:

simple sequence repeat

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Acknowledgements

The authors thank Drs Shaobin Zhong and Robert McIntosh for critical review of the manuscript. This research was support by USDA-ARS CRIS project 5442–22000–033.

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Authors and Affiliations

  1. USDA-ARS Cereal Crops Research Unit, Red River Valley Agricultural Research Center, Fargo, ND, 58105, USA

    Justin D. Faris, Steven S. Xu & Timothy L. Friesen

  2. USDA-ARS, Northern Crop Science Laboratory, 1307 18th Street North, Fargo, ND, 58105–5677, USA

    Justin D. Faris, Steven S. Xu & Timothy L. Friesen

  3. Department of Plant Sciences, Loftsgard Hall, North Dakota State University, Fargo, ND, 58105, USA

    Xiwen Cai

  4. USDA-ARS Cereal Disease Laboratory, University of Minnesota, St. Paul, MN, 55108, USA

    Yue Jin

Authors
  1. Justin D. Faris
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  2. Steven S. Xu
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  3. Xiwen Cai
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  4. Timothy L. Friesen
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  5. Yue Jin
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Correspondence to Justin D. Faris.

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Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture.

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Faris, J.D., Xu, S.S., Cai, X. et al. Molecular and cytogenetic characterization of a durum wheat–Aegilops speltoides chromosome translocation conferring resistance to stem rust. Chromosome Res 16, 1097–1105 (2008). https://doi.org/10.1007/s10577-008-1261-3

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  • DOI: https://doi.org/10.1007/s10577-008-1261-3

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