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RhoGDI2 Expression in Astrocytes After an Excitotoxic Lesion in the Mouse Hippocampus

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Abstract

The Rho GDP-dissociation inhibitor (RhoGDI) originally downregulates Rho family GTPases by preventing nucleotide exchange and membrane association. Although RhoGDI2 functions as a metastasis regulator, little is known in glial cells under neuropathological conditions. We monitored RhoGDI2 expression in the mouse brain after administering a kainic acid(KA)-induced excitotoxic lesion. In control, RhoGDI2 immunoreactivity (IR) was evident in the neuronal layer of the hippocampus. However, RhoGDI2 IR was increased in astrocytes markedly throughout the hippocampus at day 3 post-treatment with KA. To further investigate the molecular mechanism of RhoGDI2-induced cellular migration, primary astrocytes were transfected with the flag-tagged RhoGDI2 cDNA. Cell migration assay revealed that RhoGDI2 cDNA transfection inhibits astrocyte migration. Overexpression of RhoGDI2 leads to inhibit protein kinase B (PKB) activation and cdc42 and cAMP-responsive element-binding protein (CREB) phosphorylation. In conclusion, our results suggested for the first time that RhoGDI2 is required for PKB and CREB activation and cdc42 expression in astrocyte migration after KA-mediated excitotoxic lesion in mouse brain.

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Acknowledgments

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning (2013R1A1A1A05006966) and by Chungnam National University Hospital Research Fund, 2013.

Conflict of interest

The authors declare no financial or other conflicts of interest related to this study.

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Authors and Affiliations

  1. Department of Anatomy, Brain Research Institute, Chungnam National University School of Medicine, Daejeon, 301-747, South Korea

    Min-Hee Yi, Enji Zhang & Dong Woon Kim

  2. Department of Biomedical Laboratory Science, College of Health & Welfare, Kyungwoon University, Gumi, 730-739, Korea

    Kisang Kwon

  3. Department of Anatomy, School of Medicine, Chungbuk National University, Cheongju, 361-763, South Korea

    Je Hoon Seo

  4. Department of Anatomy and Neurobiology, Institute of Health Sciences, Medical Research Center for Neural Dysfunction, School of Medicine, Gyeongsang National University, Jinju, 660-702, South Korea

    Sang Soo Kang

  5. Liver and Immunology Research Center, Korean Medical College of Daejeon University, Daejeon, 300-716, South Korea

    Chang-Gue Son

  6. Department of Pediatrics, Chungnam National University Hospital, Daejeon, 301-721, South Korea

    Joon Won Kang

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  1. Min-Hee Yi
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  2. Kisang Kwon
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  3. Enji Zhang
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  4. Je Hoon Seo
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  5. Sang Soo Kang
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  6. Chang-Gue Son
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  7. Joon Won Kang
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  8. Dong Woon Kim
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Corresponding authors

Correspondence to Joon Won Kang or Dong Woon Kim.

Additional information

Min-Hee Yi and Kisang Kwon have contributed equally to this work.

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Yi, MH., Kwon, K., Zhang, E. et al. RhoGDI2 Expression in Astrocytes After an Excitotoxic Lesion in the Mouse Hippocampus. Cell Mol Neurobiol 35, 167–174 (2015). https://doi.org/10.1007/s10571-014-0108-z

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  • DOI: https://doi.org/10.1007/s10571-014-0108-z

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