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Membrane Organization and Function of the Serotonin1A Receptor

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Abstract

(1) The serotonin1A receptor is a G-protein coupled receptor involved in several cognitive, behavioral, and developmental functions. It binds the neurotransmitter serotonin and signals across the membrane through its interactions with heterotrimeric G-proteins. (2) Lipid–protein interactions in membranes play an important role in the assembly, stability, and function of membrane proteins. The role of membrane environment in serotonin1A receptor function is beginning to be addressed by exploring the consequences of lipid manipulations on the ligand binding and G-protein coupling of serotonin1A receptors, the ability to functionally solubilize the serotonin1A receptor, and the factors influencing the membrane organization of the serotonin1A receptor. (3) Recent developments involving the application of detergent-based and detergent-free approaches to understand the membrane organization of the serotonin1A receptor under conditions of ligand activation and modulation of membrane lipid content, with an emphasis on membrane cholesterol, are described.

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Acknowledgments

Work in A.C.’s laboratory was supported by the Council of Scientific and Industrial Research, Department of Biotechnology, Life Sciences Research Board, and the International Society for Neurochemistry. A.C. is an Honorary Faculty Member of the Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore (India). Some of the work described in this article was carried out by former and present members of A.C.’s research group whose contributions are gratefully acknowledged. We thank members of the A.C. laboratory for critically reading the manuscript.

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  1. Shanti Kalipatnapu

    Present address: Division of Biological Sciences, Section of Molecular Biology, University of California, San Diego, La Jolla, CA, 92093-0377, USA

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  1. Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad, 500 007, India

    Shanti Kalipatnapu & Amitabha Chattopadhyay

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  1. Shanti Kalipatnapu
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  2. Amitabha Chattopadhyay
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Correspondence to Amitabha Chattopadhyay.

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Kalipatnapu, S., Chattopadhyay, A. Membrane Organization and Function of the Serotonin1A Receptor. Cell Mol Neurobiol 27, 1097–1116 (2007). https://doi.org/10.1007/s10571-007-9189-2

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  • DOI: https://doi.org/10.1007/s10571-007-9189-2

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