Abstract
Background
The genes mutated in the cancer-prone syndrome, xeroderma pigmentosum (XP genes), have been well studied both biochemically and mechanistically. These genes are important components of the DNA nucleotide excision repair (NER) pathway, which protects against environmentally-induced cancers. XP genes are also downstream of the hereditary breast cancer syndrome gene, BRCA1, suggesting that XP genes may be important to hereditary forms of breast cancer as well. Although mutated XP genes are rare, polymorphic forms with potential functional deficiencies are common, and could pose a significant cancer risk in the general population.
Hypothesis
This study tested the hypothesis that common polymorphic variants of XP genes were associated with the risk of breast cancer among a population of women in Washington County, Maryland.
Methods
Five single nucleotide polymorphisms (SNPs) among four XP genes (XPC, XPD, XPF and XPG) were genotyped from DNA samples collected at baseline, and then analyzed by conditional logistic regression for association with the incidence of breast cancer. 321 cases were individually matched to 321 controls, by age and menopausal status.
Results
No significant associations were found between breast cancer risk and any of the XP genotypes. Odds ratios for all genotypes ranged from 0.61 to 1.14, and none were statistically significant. Adjustment and stratification for family history of breast cancer did not alter the findings.
Conclusion
These results suggest that polymorphisms of XP genes are not likely to be significant risk factors for women within the general population. This study did not address, however, risks for subpopulations of women with high exposures to DNA damaging agents.
Similar content being viewed by others
References
Lindahl T, Wood RD (1999) Quality control by DNA repair. Science 286:1897–1905
Kraemer KH, Levy DD, Parris CN, Gozukara EM, Moriwaki S, Adelberg S, Seidman MM (1994) Xeroderma pigmentosum and related disorders: examining the linkage between defective DNA repair and cancer. J Invest Dermatol 103 Suppl. 5:96S–101S
Kraemer KH, Lee M-M, Andrews AD, Lambert WC (1994) The role of sunlight and DNA repair in melanoma and nonmelanoma skin cancer: The xeroderma pigmentosum paradigm. Arch Dermatol 130:1018–1021
Somasundaram K (2003) Breast cancer gene 1 (BRCA1): role in cell cycle regulation and DNA repair—perhaps through transcription. J Cell Biochem 88(6):1084–1091
El-Deiry WS (2002) Transactivation of repair genes by BRCA1. Cancer Biol Ther 1(5):490–491
Reardon JT, Sancar A (2005) Nucleotide excision repair. Prog Nucleic Acid Res Mol Biol 79:183–235
Helzlsouer KJ, Alberg AJ, Huang HY, Hoffman SC, Strickland PT, Brock JW, Burse VW, Needham LL, Bell DA, Lavigne JA, etal (1999) Serum concentrations of organochlorine compounds and the subsequent development of breast cancer. Cancer Epidemiol Biomarkers Prev 8(6):525–532
Klintschar M, Neuhuber F (2000) Evaluation of an alkaline lysis method for the extraction of DNA from whole blood and forensic stains for STR analysis. J Forensic Sci 45(3):669–673
Rubin DB (1996) Multiple imputation after 18+ years. J Am Stat Assoc 91(434):473–489
Schafer JL (1997) Analysis of incomplete multivariate data. vol. 72. Chapman & Hall, New York
Greenland S, Finkle WD (1995) A critical look at methods for handling missing covariates in epidemiologic regression analyses. Am J Epidemiol 142(12):1255–1264
Brewster AM, Jorgensen TJ, Ruczinski I, Huang HY, Hoffman S, Thuita L, Newschaffer C, Lunn RM, Bell D, Helzlsouer KJ (2006) Polymorphisms of the DNA repair genes XPD (Lys751Gln) and XRCC1 (Arg399Gln and Arg194Trp): relationship to breast cancer risk and familial predisposition to breast cancer. Breast Cancer Res Treat 95(1):73–80
Pharoah PD, Dunning AM, Ponder BA, Easton DF (2004) Association studies for finding cancer-susceptibility genetic variants. Nat Rev Cancer 4(11):850–860
Lehmann AR (2003) DNA repair-deficient diseases, xeroderma pigmentosum, Cockayne syndrome and trichothiodystrophy. Biochimie 85(11):1101–1111
Amiel A, Peretz G, Slor H, Weinstein G, Fejgin MD (2004) Molecular cytogenetic parameters in fibroblasts from patients and carriers of xeroderma pigmentosum. Cancer Genet Cytogenet 149(2):154–160
Cheo DL, Meira LB, Burns DK, Reis AM, Issac T, Friedberg EC (2000) Ultraviolet B radiation-induced skin cancer in mice defective in the Xpc, Trp53, and Apex (HAP1) genes: genotype-specific effects on cancer predisposition and pathology of tumors. Cancer Res 60(6):1580–1584
Antoniou AC, Pharoah PD, McMullan G, Day NE, Ponder BA, Easton D (2001) Evidence for further breast cancer susceptibility genes in addition to BRCA1 and BRCA2 in a population-based study. Genet Epidemiol 21(1):1–18
Antoniou AC, Pharoah PD, McMullan G, Day NE, Stratton MR, Peto J, Ponder BJ, Easton DF (2002) A comprehensive model for familial breast cancer incorporating BRCA1, BRCA2 and other genes. Br J Cancer 86(1):76–83
Goode EL, Ulrich CM, Potter JD (2002) Polymorphisms in DNA repair genes and associations with cancer risk. Cancer Epidemiol Biomarkers Prev 11(12):1513–1530
Zhang L, Zhang Z, Yan W (2005) Single nucleotide polymorphisms for DNA repair genes in breast cancer patients. Clin Chim Acta 359(1–2):150–155
Spillare EA, Wang XW, von Kobbe C, Bohr VA, Hickson ID, Harris CC (2005) Redundancy of DNA helicases in p53-mediated apoptosis. Oncogene 25(14):2119–2123
Seker H, Butkiewicz D, Bowman ED, Rusin M, Hedayati M, Grossman L, Harris CC (2001) Functional significance of XPD polymorphic variants: attenuated apoptosis in human lymphoblastoid cells with the XPD 312 Asp/Asp genotype. Cancer Res 61(20):7430–7434
Rothkamm K, Lobrich M (2003) Evidence for a lack of DNA double-strand break repair in human cells exposed to␣very low X-ray doses. Proc Natl Acad Sci USA 100(9):5057–5062
Terry MB, Gammon MD, Zhang FF, Eng SM, Sagiv SK, Paykin AB, Wang Q, Hayes S, Teitelbaum SL, Neugut AI, etal (2004) Polymorphism in the DNA repair gene XPD, polycyclic aromatic hydrocarbon-DNA adducts, cigarette smoking, and breast cancer risk. Cancer Epidemiol Biomarkers Prev 13(12):2053–2058
Smith TR, Levine EA, Perrier ND, Miller MS, Freimanis RI, Lohman K, Case LD, Xu J, Mohrenweiser HW, Hu JJ (2003) DNA-repair genetic polymorphisms and breast cancer risk. Cancer Epidemiol Biomarkers Prev 12(11 Pt 1):1200–1204
Kumar R, Hoglund L, Zhao C, Forsti A, Snellman E, Hemminki K (2003) Single nucleotide polymorphisms in the XPG gene: determination of role in DNA repair and breast cancer risk. Int J Cancer 103(5):671–675
Forsti A, Angelini S, Festa F, Sanyal S, Zhang Z, Grzybowska E, Pamula J, Pekala W, Zientek H, Hemminki K, etal (2004) Single nucleotide polymorphisms in breast cancer. Oncol Rep 11(4):917–922
Acknowledgements
Grant Support: Research was funded by a National Cancer Institute Breast Spore Award (P50 CA88843), and the National Institute of Aging 5U01AG018033. T.J.J. was funded by an NIH Ruth L. Kirschstein Senior Fellow Award (NCI F33 CA09817-01). K.V. is a recipient an ASCO Career Development Award and an NIH Preventive Oncology Academic Award (NCI K07 CA111948). I.R. was supported, in part, by the Maryland Cigarette Restitution Fund Research Grant to the Johns Hopkins Medical Institutions and NIH grant CA 074841.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Jorgensen, T.J., Visvanathan, K., Ruczinski, I. et al. Breast Cancer Risk is not Associated with Polymorphic Forms of Xeroderma Pigmentosum Genes in a Cohort of Women from Washington County, Maryland. Breast Cancer Res Treat 101, 65–71 (2007). https://doi.org/10.1007/s10549-006-9263-3
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10549-006-9263-3