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Characterization of multiple novel aerobic polychlorinated biphenyl (PCB)-utilizing bacterial strains indigenous to contaminated tropical African soils

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Abstract

Contaminated sites in Lagos, Nigeria were screened for the presence of chlorobiphenyl-degrading bacteria. The technique of continual enrichment on Askarel fluid yielded bacterial isolates able to utilize dichlorobiphenyls (diCBs) as growth substrates and six were selected for further studies. Phenotypic typing and 16S rDNA analysis classified these organisms as species of Enterobacter, Ralstonia and Pseudomonas. All the strains readily utilized a broad spectrum of xenobiotics as sole sources of carbon and energy. Growth was observed on all monochlorobiphenyls (CBs), 2,2′-, 2,3-, 2,4′-, 3,3′- and 3,5-diCB as well as di- and trichlorobenzenes Growth was also sustainable on Askarel electrical transformer fluid and Aroclor 1221. Time-course studies using 100 ppm of 2-, 3- or 4-CB resulted in rapid exponential increases in cell numbers and CB transformation to respective chlorobenzoates (CBAs) within 70 h. Significant amounts of chloride were recovered in culture media of cells incubated with 2-CB and 3-CB, suggesting susceptibilities of both 2- and 3-chlorophenyl rings to attack, while the 4-CB was stoichiometrically transformed to 4-CBA. Extensive degradation of most of the congeners in Aroclor 1221 was observed when isolates were cultivated with the mixture as a sole carbon source. Aroclor 1221 was depleted by a minimum of 51% and maximum of 71%. Substantial amounts of chloride eliminated from the mixture ranged between 15 and 43%. These results suggest that some contaminated soils in the tropics may contain exotic micro-organisms whose abilities and potentials are previously unknown. An understanding of these novel strains therefore, may help answer questions about the microbial degradation of polychlorinated biphenyls (PCBs) in natural systems and enhance the potential use of bioremediation as an effective tool for cleanup of PCB-contaminated soils.

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Acknowledgements

Authors would like to acknowledge the financial support of the ICSC-World Laboratory, Lausanne, Switzerland and the School of Public and Environmental Affairs, Indiana University, Bloomington, IN, USA. We thank Nathan Grindle for assistance with 16S rRNA gene sequence analysis and the anonymous reviewers for helpful comments that improved the manuscript.

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Authors and Affiliations

  1. Department of Botany and Microbiology, Faculty of Science, University of Lagos, Akoka, Yaba, Lagos, Nigeria

    Sunday A. Adebusoye, Matthew O. Ilori & Olukayode O. Amund

  2. Environmental Science Research Center, School of Public and Environmental Affairs, Indiana University, Bloomington, IN, 47405, USA

    Sunday A. Adebusoye & Flynn W. Picardal

  3. Department of Biological Sciences, College of Art and Sciences, Indiana University, Bloomington, IN, 47405, USA

    Clay Fuqua

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  1. Sunday A. Adebusoye
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  2. Flynn W. Picardal
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  3. Matthew O. Ilori
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  4. Olukayode O. Amund
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  5. Clay Fuqua
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Correspondence to Sunday A. Adebusoye.

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Adebusoye, S.A., Picardal, F.W., Ilori, M.O. et al. Characterization of multiple novel aerobic polychlorinated biphenyl (PCB)-utilizing bacterial strains indigenous to contaminated tropical African soils. Biodegradation 19, 145–159 (2008). https://doi.org/10.1007/s10532-007-9122-x

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  • DOI: https://doi.org/10.1007/s10532-007-9122-x

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