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Reproductive isolation and the expansion of an invasive hybrid swarm

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Abstract

Biological invasions involving hybridization proceed according to prezygotic and postzygotic reproductive isolating mechanisms. Yet few comparisons of reproductive isolation have been carried out to understand how different mechanisms prevent or promote invasions involving hybridization. Here we present a study of prezygotic and postzygotic isolation between non-native red shiner (Cyprinella lutrensis) and native blacktail shiner (C. venusta stigmatura) from the Coosa River basin (USA) to better understand the formation and expansion of invasive hybrid swarms. We conducted spawning trials to measure mating preferences and raised broods from crosses to assay hybrid viability through early juvenile development. Females of both species were more responsive to conspecific mates, although blacktail shiner females responded more often to heterospecific mates than did red shiner females. Fecundity of red shiner females was also higher than blacktail shiner females. Heterospecific crosses resulted in lower fertilization and egg hatching rates, but we found no other evidence of inviability. Rather, we found comparatively low larval mortality of F1 hybrids, which is suggestive of heterosis. These findings support prior inferences of assortative mating from genetic descriptions of hybridization, and that the invasion in the Coosa River is likely proceeding due to interspecific competition and intrinsic hybrid viability.

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Acknowledgments

We thank J. Fontaine and G. Glotzbecker for assisting with the care and maintenance of fishes; K. Blocksom for assisting with the statistical analyses; C. Ferguson, S. Snider and an anonymous reviewer for editorial assistance; and J. Fontaine, E. Derryberry, R. Hamilton, C. Storey, and C. Tepolt for assisting with field collections. Although this work was reviewed by the US EPA and approved for publication, it may not necessarily reflect official Agency policy.

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Authors and Affiliations

  1. National Exposure Research Laboratory, US Environmental Protection Agency, Cincinnati, OH, 45268, USA

    Michael J. Blum & David M. Walters

  2. Department of Ecology and Evolutionary Biology, Tulane University, New Orleans, LA, 70118, USA

    Michael J. Blum

  3. Fort Collins Science Center, US Geological Survey, Fort Collins, CO, 80526, USA

    David M. Walters

  4. Florida Integrated Science Center, US Geological Survey, Gainesville, FL, 32653, USA

    Noel M. Burkhead

  5. Georgia Museum of Natural History and Odum School of Ecology, University of Georgia, Athens, GA, 30602, USA

    Byron J. Freeman

  6. Department of Biological Sciences, Duquesne University, Pittsburgh, PA, 15282, USA

    Brady A. Porter

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  1. Michael J. Blum
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Correspondence to Michael J. Blum.

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Blum, M.J., Walters, D.M., Burkhead, N.M. et al. Reproductive isolation and the expansion of an invasive hybrid swarm. Biol Invasions 12, 2825–2836 (2010). https://doi.org/10.1007/s10530-010-9688-9

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