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Oceanobacillus timonensis sp. nov. and Oceanobacillus senegalensis sp. nov., two new moderately halophilic, Gram-stain positive bacteria isolated from stools sample of healthy young Senegalese

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Abstract

Oceanobacillus timonensis Marseille-P3532T (CSUR P3532, CCUG 70981) and Oceanobacillus senegalensis Marseille-P3587T (CSUR P3587, CCUG 70613), are the type strains of O. timonensis sp. nov. and O. senegalensis sp. nov., respectively. They are moderately halophilic, aerobic, motile and Gram-stain positive bacteria. The strains P3532T and P3587T were isolated from stools with 3.8% and 2.1% sodium chloride (NaCl) of healthy 10 year old female and male 7-year-old children, respectively and living respectively at Dielmo and N’diop two villages in Senegal (West Africa). This study aimed to describe the genome and phenotypic characteristics of O. timonensis Marseille-P3532T and O. senegalensis Marseille-P3587T. The genomes are 4,485,335 bp long for O. timonensis and 4,300,331 bp for O. senegalensis with 38.78% and 36.92% G+C content, respectively. They contain 4306 and 3979 protein-coding and 87 and 273 RNAs genes, respectively.

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Acknowledgements

The authors thank the Xegen Company (www.xegen.fr) for automating the genomic annotation process. This work was carried out thanks to the support of the Méditerranée Infection Foundation and the National Research Agency under the program “Investissements d’avenir,” reference ANR-10-IAHU-03.

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Authors and Affiliations

  1. Aix Marseille Univ, IRD, AP-HM, MEPHI, IHU Méditerranée Infection, Marseille, France

    Bruno Senghor, Saber Khelaifia, Didier Raoult & Jean-Christophe Lagier

  2. Fondation Méditerranée Infection, IHU Méditerranée Infection, Marseille, France

    Bruno Senghor

  3. Aix Marseille Univ, IRD, SSA, AP-HM, VITROME, IHU Méditerranée Infection, Marseille, France

    Hubert Bassène, Catherine Robert, Pierre-Edouard Fournier & Cheikh Sokhna

  4. Department of Bacteriology, Nice Academic Hospital, Nice, France

    Raymond Ruimy

  5. Nice-Sophia Antipolis University, INSERM U1065 (C3M), Bacterial Toxins in Host Pathogen Interactions, C3M, Bâtiment Universitaire, Archimed, Nice, France

    Raymond Ruimy

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  1. Bruno Senghor
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Contributions

DR, JCL, CS and RR: study conception; BS, HB and SK: Stool collection, culture and phenotypic experiments; CR, PEF: genome sequencing and analysis; BS and JCL wrote the manuscript. CS, RR, HB, SK, CR and PEF: reviewing the manuscript.

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Correspondence to Jean-Christophe Lagier.

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Senghor, B., Bassène, H., Khelaifia, S. et al. Oceanobacillus timonensis sp. nov. and Oceanobacillus senegalensis sp. nov., two new moderately halophilic, Gram-stain positive bacteria isolated from stools sample of healthy young Senegalese. Antonie van Leeuwenhoek 112, 785–796 (2019). https://doi.org/10.1007/s10482-018-01212-0

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