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Comparison of Artery Organ Culture and Co-culture Models for Studying Endothelial Cell Migration and Its Effect on Smooth Muscle Cell Proliferation and Migration

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Abstract

Arterial restenosis associated with intimal hyperplasia is the major cause of long-term failure of vascular interventions. Endothelium injury and the proliferation and migration of smooth muscle cells (SMC) are key events in the development of intimal hyperplasia. The objectives of this study were to develop an ex vivo artery injury model for studying endothelial cell (EC) migration and to compare it with an in vitro co-culture arterial wall injury model in terms of the effect of flow on EC migration and its effect on SMC migration and proliferation. Our results demonstrated that shear flow improves reendothelialization in the injured area by promoting EC migration. The migration distance of ECs is much smaller in the arteries than in an in vitro cell culture model (3.57 ± 1.29 mm vs. 5.2 ± 1.4 cm, p < 0.001). SMC proliferation was significantly less in the EC intact and reendothelialization areas than in the EC denuded areas indicating that reendothelialization suppresses SMC proliferation. Our models provide a new approach to study techniques to enhance endothelium healing.

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Acknowledgments

This work was supported by the Advanced Research Program from the Texas Higher Educational Coordinating Board through grant # 003659-0014-2006. It was also partially supported by an MBRS-SCORE pilot grant from the National Institute of Health through grant # S06GM008194 and grant # 0602834 from the National Science Foundation. The authors thank the Granzins at New Braunfels and Wiatrek at Poth, TX for generously providing the arteries for this work. The authors also thank Mr. Kurtis Johnson for his help in this work and Ms. Patricia Navarro for proofreading the manuscript.

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Authors and Affiliations

  1. UTSA/UTHSCSA Biomedical Engineering Program, San Antonio, TX, USA

    Yong-Ung Lee, Eugene Sprague & Hai-Chao Han

  2. Department of Radiology, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA

    Jian Luo & Eugene Sprague

  3. Department of Mechanical Engineering, University of Texas at San Antonio, San Antonio, TX, 78249, USA

    Hai-Chao Han

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  1. Yong-Ung Lee
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Correspondence to Hai-Chao Han.

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Associate Editor Julia E. Babensee oversaw the review of this article.

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Lee, YU., Luo, J., Sprague, E. et al. Comparison of Artery Organ Culture and Co-culture Models for Studying Endothelial Cell Migration and Its Effect on Smooth Muscle Cell Proliferation and Migration. Ann Biomed Eng 38, 801–812 (2010). https://doi.org/10.1007/s10439-009-9877-9

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