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Osteoblast Elastic Modulus Measured by Atomic Force Microscopy Is Substrate Dependent

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Abstract

The actin and microtubule cytoskeleton have been found to contribute to the elastic modulus of cells, which may be modulated by adhesion to extracellular matrix (ECM) proteins and subsequent alterations in the cytoskeleton. In this study, the apparent elastic modulus (Eapp) of osteoblast-like MC3T3-E1 cells adhered to fibronectin (FN), vitronectin (VN), type I collagen (COLI), fetal bovine serum (FBS), or poly-l-lysine (PLL), and bare glass were determined using an atomic force microscope (AFM). The Eapp of osteoblasts adhered to ECM proteins (FN, VN, COLI, and FBS) that bind cells via integrins were higher compared to cells on glass and PLL, which adhere cells through nonspecific binding. Also, osteoblasts adhered to FN, VN, COLI, and FBS had F-actin stress fiber formation, while osteoblasts on glass and PLL showed few F-actin fibers. Disruption of the actin cytoskeleton decreased Eapp of osteoblasts plated on FN to the level of osteoblasts plated on glass, while microtubule disruption had no significant effect. This suggests that the elevated modulus of osteoblasts adhered to FN was due to remodeling of the actin cytoskeleton upon adhesion to ECM proteins. Modulation of cell stiffness upon adhesion to various substrates may influence mechanosignal transduction in osteoblasts.

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Authors and Affiliations

  1. Bone Bioengineering Laboratory, Columbia University, New York, NY

    Erica Takai & X. Edward Guo

  2. Cardiac Cell Mechanics Laboratory, Columbia University, New York, NY

    Kevin D. Costa & Aisha Shaheen

  3. Department of Biomedical Engineering, Cellular Engineering Laboratory, Columbia University, New York, NY

    Clark T. Hung

  4. 351 Engineering Terrace, Columbia University, MC 8904, 1210 Amsterdam Ave, New York, NY, 10027

    X. Edward Guo

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Takai, E., Costa, K.D., Shaheen, A. et al. Osteoblast Elastic Modulus Measured by Atomic Force Microscopy Is Substrate Dependent. Ann Biomed Eng 33, 963–971 (2005). https://doi.org/10.1007/s10439-005-3555-3

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