Skip to main content

Advertisement

SpringerLink
Log in

Impact of the Carpathians on the genetic structure of the spruce bark beetle Ips typographus

  • Original Paper
  • Published:
Journal of Pest Science Aims and scope Submit manuscript

Abstract

The Carpathians are a range of mountains forming an arc roughly 1,500 km long across Central and Eastern Europe. They are an important area for biodiversity and belong to one of the major refuges of the last ice ages for many organisms. The forests of the Carpathians are dominated by spruce, which have suffered continuous outbreaks of the eight spined spruce bark beetle, Ips typographus, in recent decades. The phylogeography of this spruce pest is well documented, however, little is known on small scale, i.e., the Carpathians. Here we applied a mitochondrial marker and studied the genetic variation and structure of Carpathian populations and compared data with published one from other European populations. Twelve haplotypes were characterized and 42 % of those were not detected in other European populations. Despite a slight genetic structure, differences were observed in the haplotype distribution and diversity between the Western/Southern Carpathians and the Eastern Carpathians reflecting at least two potential refugial areas for I. typographus within the Carpathian mountain system. Further data show that the Eastern Beskidian Mountains of the Carpathians could act as barrier for several European haplotypes. This small-scale analysis reveals that the Carpathians have been an important glacial and post-glacial refuge for I. typographus. This information is important for a preventive and reactive forest management.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Avtzis DN, Bertheau C, Stauffer C (2012) What is next in bark beetle phylogeography? Insects 3:453–472

    Article  Google Scholar 

  • Babik W, Branicki W, Crnobrnja-Isailovic J, Cogalniceanu D, Sas I, Olgun K, Poyarkov NA, Garcia-París M, Arntzen JW (2005) Phylogeography of two European new species-discordance between mtDNA and morphology. Mol Ecol 14:2475–2491

    Article  PubMed  CAS  Google Scholar 

  • Bertheau C, Krumböck S, Schuler H, Arthofer W, Stauffer C (2011) Hit or miss in phylogeographic analyses: the case of the cryptic NUMTs. Mol Ecol Resour 11:1056–1059

    Article  PubMed  Google Scholar 

  • Bertheau C, Schuler H, Arthofer W, Avtzis D, Mayer F, Krumböck S, Moodley Y, Stauffer C (2013) Divergent evolutionary histories of two sympatric spruce bark beetle species. Mol Ecol. doi:10.1111/mec.12296

  • Botterweg PF (1982) Dispersal and flight behaviour of the spruce bark beetle Ips typographus in relation to sex, size and fat content. Z Angew Entomol 94:466–489

    Article  Google Scholar 

  • Clement M, Posada D, Crandall K (2000) TCS: a computer program to estimate gene genealogies. Mol Ecol 9:1657–1660

    Article  PubMed  CAS  Google Scholar 

  • Conord C, Lempérière G, Taberlet P, Després L (2006) Genetic structure of the forest pest Hylobius abietis on conifer plantations at different spatial scales in Europe. Heredity 97:46–55

    Article  PubMed  CAS  Google Scholar 

  • Duelli P, Zahradnik P, Knizek M, Kalinova B (1997) Migration in spruce bark beetles (Ips typographus L.) and the efficiency of pheromone traps. J Appl Entomol 121:297–303

    Article  Google Scholar 

  • Excoffier L, Laval G, Schneider S (2005) Arlequin (version 3.0) an integrated software package for population genetics data analysis. Evol Bioinform 1:47–50

    CAS  Google Scholar 

  • Excoffier L, Smouse PE, Quattro JM (1992) Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data. Genetics 131:479–491

    PubMed  CAS  Google Scholar 

  • Faccoli M, Stergulc F (2004) Ips typographus (L.) pheromone trapping in south Alps: spring catches determine damage thresholds. J Appl Entomol 128:307–311

    Article  Google Scholar 

  • Felsenstein J (1988) Phylogenies from molecular sequences: inference and reliability. Annu Rev Genet 22:521–565

    Article  PubMed  CAS  Google Scholar 

  • Forsse E, Solbreck C (1985) Migration in the bark beetle Ips typographus L.: duration timing and height of flight. Z Angew Entomol 100:47–57

    Article  Google Scholar 

  • Grégoire JC, Evans H (2004) Damage and control of BAWBILT organisms: an overview. In: Lieutier F et al (eds) Bark and wood boring insects in living trees in Europe, a synthesis. Kluwer, Dordrecht, pp 19–37

    Chapter  Google Scholar 

  • Gries G (1985) Zur Frage der Dispersion des Buchdruckers (Ips typographus L.). Z Angew Entomol 99:12–20

    Article  Google Scholar 

  • Gurung AB, Bokwa A, Chełmicki W, Elbakidze M, Hirschmugl M, Hostert P et al (2009) Global change research in the Carpathian mountain region. Mt Res Dev 29:282–288

    Article  Google Scholar 

  • Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl Acid 41:95–98

    CAS  Google Scholar 

  • Hasegawa M, Kishino H, Yano TA (1985) Dating of the human–ape splitting by a molecular clock of mitochondrial DNA. J Mol Evol 22:160–174

    Article  PubMed  CAS  Google Scholar 

  • Jaarola M, Searle JB (2002) Phylogeography of field voles (Microtus agrestis) in Eurasia inferred from mitochondrial DNA sequences. Mol Ecol 11:2613–2621

    Article  PubMed  CAS  Google Scholar 

  • Jakus R, Zajíčkova L, Cudlín P, Blaženec M, Turčani M, Ježík M, Lieutier F, Schlyter F (2011) Landscape-scale Ips typographus attack dynamics: from monitoring plots to GIS-based disturbance models. Forest 4:256–261

    Google Scholar 

  • Juan C, Oromi P, Hewitt GM (1995) Mitochondrial DNA phylogeny and sequential colonization of Canary Islands by darkling beetles of the genus Pimelia (Tenebrionidae). Proc R Soc Lond B Biol Sci 261:173–180

    Article  CAS  Google Scholar 

  • King RA, Ferris C (1998) Chloroplast DNA phylogeography of Alnus glutinosa (L.) Gaertn. Mol Ecol 7:1151–1161

    Article  CAS  Google Scholar 

  • Kotlik P, Deffontaine V, Mascheretti S, Zima J, Michaux JR, Searle JB (2006) A northern glacial refugium for bank voles (Clethrionomys glareolus). Proc Natl Acad Sci U S A 103:1860–1864

    Article  Google Scholar 

  • Lieutier F (2002) Mechanisms of resistance in conifers and bark beetle attack strategies. In: Wagner MR, Clancy KM, Lieutier F, Paine TD (eds) Mechanisms and deployment of resistance in trees to insects. Kluwer Academic Publishers, Dordrecht, pp 31–77

    Chapter  Google Scholar 

  • Lieutier F, Day KR, Battisti A, Grégoire JC, Evans HF (2004) Bark and wood boring insects in living trees in Europe, a synthesis. Kluwer Academic Publishers, Dordrecht

    Book  Google Scholar 

  • Lunt D, Zhang DX, Szymura JM, Hewit GM (1996) The insect COI gene: evolutionary patterns and conserved primers for phylogenetic studies. Insect Mol Biol 5:153–165

    Article  PubMed  CAS  Google Scholar 

  • Magri D, Vendramin GG, Comps B, Dupanloup I, Geburek T, Gomory D et al (2006) A new scenario for the Quaternary history of European beech populations: palaeobotanical evidence and genetic consequences. New Phytol 171:199–221

    Article  PubMed  CAS  Google Scholar 

  • Mardulyn P, Mikhailov YE, Pasteels JM (2009) Testing phylogeographic hypotheses in a Euro-Siberian cold-adapted leaf beetle with coalescent simulations. Evolution 63:2717–2729

    Article  PubMed  CAS  Google Scholar 

  • McCarthy C (1996) Chromas ver. 1.45. School of Health Science, Griffith University, Southport

    Google Scholar 

  • Mraz P, Gaudeul M, Rioux D, Gielly L, Choler P, Taberlet P (2007) Genetic structure of Hypochaeris uniflora (Asteraceae) suggests vicariance in the Carpathians and rapid post-glacial colonization of the Alps from an eastern Alpine refugium. J Biogeogr 34:2100–2114

    Article  Google Scholar 

  • Pauls SU, Lumbsch HT, Haase P (2006) Phylogeography of the montane caddisfly Drusus discolor: evidence for multiple refugia and periglacial survival. Mol Ecol 15:2153–2169

    Article  PubMed  CAS  Google Scholar 

  • Pecskay Z, Lexa J, Szakacs A, Seghedi I, Balogh K, Konecny V et al (2006) Geochronology of Neogene magmatism in the Carpathian arc and intra-Carpathian area. Geol Carpath 57:511–530

    Google Scholar 

  • Peterson MA, Denno RF (1998) The influence of dispersal and diet breadth on patterns of genetic isolation by distance in phytophagous insects. Am Nat 152:428–446

    Article  PubMed  CAS  Google Scholar 

  • Petit R, El Mousadik A, Pons O (1998) Identifying populations for conservation on the basis of genetic markers. Conserv Biol 12:844–855

    Article  Google Scholar 

  • Pons O, Petit RJ (1996) Measuring and testing genetic differentiation with ordered versus unordered alleles. Genetics 144:1237–1245

    PubMed  CAS  Google Scholar 

  • Posada D, Crandall KA (1998) MODELTEST: testing the model of DNA substitution. Bioinformatics 14:817–818

    Article  PubMed  CAS  Google Scholar 

  • Ronikier M, Cieślak E, Korbecka G (2008) High genetic differentiation in the Alpine plant Campanula alpina: evidence for glacial survival in several Carpathian regions and long-term isolation between the Carpathians and the Eastern Alps. Mol Ecol 17:1763–1775

    Article  PubMed  CAS  Google Scholar 

  • Ruffini VF, Streifeneder T, Eiselt B (2006) Implementing an international mountain convention, an approach for the delimitation of the Carpathian Convention area. Eurac-research and United Nations Environment Programme—Vienna Interim Secretariat of the Carpathian Convention UNEP Vienna—ISCC

  • Sallé A, Kerdelhué C, Breton M, Lieutier F (2003) Characterization of microsatellite loci in the spruce bark beetle Ips typographus (Coleoptera: Scolytinae). Mol Ecol Notes 3:336–337

    Google Scholar 

  • Sallé A, Arthofer W, Lieutier F, Stauffer C, Kerdelhué C (2007) Phylogeography of a host-specific insect: genetic structure of Ips typographus in Europe does not reflect past fragmentation of its host. Biol J Linn Soc 90:239–246

    Article  Google Scholar 

  • Schmidt-Vogt H (1977) Die Fichte. Verlag Paul Parey, Hamburg

    Google Scholar 

  • Schmitt T (2009) Biogeographical and evolutionary importance of the European high mountain systems. Front Zool 6:9

    Article  PubMed  Google Scholar 

  • Schmitt T, Seitz A (2001) Intraspecific allozymatic differentiation reveals the glacial refugia and the postglacial expansions of European Erebia medusa (Lepidoptera: Nymphalidae). Biol J Linn Soc 74:429–458

    Google Scholar 

  • Sommer RS, Benecke N (2005) The recolonization of Europe by brown bears Ursus arctos linnaeus, 1758 after the last glacial maximum. Mammal Rev 35:156–164

    Article  Google Scholar 

  • Stauffer C, Lakatos F, Hewitt GM (1999) Phylogeography and postglacial colonization routes of Ips typographus L. (Col.: Scolytidae). Mol Ecol 8:763–773

    Article  Google Scholar 

  • Stenseth NC, Kirkendall LR (1989) Population dynamics of bark beetles with special reference to Ips typographus. Holarctic Ecol 12:382–383

    Google Scholar 

  • Stoeckle BC, Kuehn R (2011) Identification of 18 polymorphic microsatellite loci in the spruce bark beetle Ips typographus L. (Coleoptera: Scolytidae) using high-throughput sequence data. Eur J Entomol 108:169–171

    CAS  Google Scholar 

  • Swofford DL (2002) PAUP*: phylogenetic analysis using parsimony (*and other methods). Sinauer Associates, Sunderland

    Google Scholar 

  • Thompson JD, Higgins DG, Gibson TJ (1994) ClustalW: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucl Acid Res 22:4673–4680

    Article  CAS  Google Scholar 

  • Tollefsrud MM, Kissling R, Gugerli F, Johnsen Ø, Skrøppa T, Cheddadi R et al (2008) Genetic consequences of glacial survival and postglacial colonization in Norway spruce: combined analysis of mitochondrial DNA and fossil pollen. Mol Ecol 17:4134–4150

    Article  PubMed  CAS  Google Scholar 

  • Ursenbacher S, Carlsson M, Helfer V, Tegelström H, Fumagalli L (2006) Phylogeography and Pleistocene refugia of the adder (Vipera berus) as inferred from mitochondrial DNA sequence data. Mol Ecol 15:3425–3437

    Article  PubMed  CAS  Google Scholar 

  • Vos P, Hogers R, Bleeker M, Reijans M, Van de Lee T, Hornes M et al (1995) AFLP: a new technique for DNA fingerprinting. Nucl Acids Res 23:4407–4414

    Article  PubMed  CAS  Google Scholar 

  • Wermelinger B (2004) Ecology and management of the spruce bark beetle Ips typographus-a review of recent research. Forest Ecol Manag 202:67–82

    Article  Google Scholar 

  • Weslien J, Lindelow A (1990) Recapture of marked spruce bark beetles (Ips typographus) in pheromone traps using area-wide mass trapping. Can J For Res 20:1786–1790

    Article  Google Scholar 

  • Zhang DX, Hewitt GM (1996) Nuclear integrations: challenges for mitochondrial DNA markers. Trends Ecol Evol 11:247–251

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This study is part of the PhD thesis of E. Krascsenitsová funded by the Operational Program of Research and Development. This project was funded by the European Fund for Regional Development (EFRD); by the Austrian Exchange Programme Aktion Österreich—Slowakei—Grant: ITMS 26220220087: The development of ecological methods to control chosen forest pests in vulnerable mountainous regions of Slovakia; by the Austrian Science Foundation FWF P21147-B17; and by the European Union Seventh Framework Programme FP7 2007–2013 (KBBE 2009-3) under grant agreement 245268 ISEFOR. We are grateful to Ján Kohout, Fedor Čiampor, Ivana Daubnerová, Veronika Michalková for their assistance in data analysis, Alena Šedivá, Susanne Krumböck, Anna Macková for their technical help, Leonard J. Beans for correcting the language and the three anonymous referees for their comments on earlier draft of the manuscript.

Author information

Authors and Affiliations

  1. Institute of Zoology, Slovak Academy of Sciences, Dúbravská cesta 9, 845 06, Bratislava, Slovakia

    Eva Krascsenitsová, Milan Kozánek & Ladislav Roller

  2. State Forests of TANAP, 059 60, Tatranská Lomnica, Slovakia

    Ján Ferenčík

  3. Department of Forest and Soil Sciences, Institute of Forest Entomology, Forest Pathology and Forest Protection, Boku, University of Natural Resources and Life Sciences, Vienna, Austria

    Christian Stauffer & Coralie Bertheau

  4. Scientica s.r.o., Hybešova 33, 831 06, Bratislava, Slovakia

    Ján Ferenčík

Authors
  1. Eva Krascsenitsová
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Milan Kozánek
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ján Ferenčík
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ladislav Roller
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Christian Stauffer
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Coralie Bertheau
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Coralie Bertheau.

Additional information

Communicated by M. Traugott.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Krascsenitsová, E., Kozánek, M., Ferenčík, J. et al. Impact of the Carpathians on the genetic structure of the spruce bark beetle Ips typographus . J Pest Sci 86, 669–676 (2013). https://doi.org/10.1007/s10340-013-0508-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10340-013-0508-8

Keywords

Search

Navigation