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Development and Validation of a Green Capillary Electrophoretic Method for Determination of Polyphenolic Compounds in Red Wine Samples

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Abstract

A sensitive, simple, rapid, experimentally convenient, cost-effective, environmentally friendly and high-throughput green chemistry by capillary electrophoresis (CE) approach for the determination of eight polyphenolics frequently found in red wines from USA was carried without using toxic organic modifier. Several parameters which affect the separation were investigated to determine the optimum conditions. At room temperature, the eight polyphenolics could be well separated within 15 min in a 55-cm length capillary at a separation voltage of 26 kV with 40-mM borate buffer (pH 8.9). The method was fully validated showing satisfactory data for all method validation parameters tested. The limits of detection varied from 0.15 to 0.32 µM. The relative standard deviations of migration varied from 0.208 to 0.630 %. The Californian red wine samples analyzed were bought in the local markets, and the polyphenolic compound recoveries were in the range of 98–99.7 %. The method was successfully applied to the determination of the studied polyphenolics in red wine samples with satisfactory recoveries. Catechin, syringic acid, apigenin, myricetin, luteolin, quercetin, caffeic acid, and gallic acid were detected in all samples, with gallic acid and myricetin occurring in the highest concentration.

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Science and Arts, Uşak University, Uşak, Turkey

    Senem Şanli

  2. Department of Food Engineering, Chemistry, Faculty of Engineering, Uşak University, Uşak, Turkey

    Nurullah Şanli

  3. Department of Analytical Chemistry, Faculty of Pharmacy, Ankara University, Tandoğan, 06100, Ankara, Turkey

    Sibel A. Ozkan

  4. Department of Chemistry, Ralph N. Adams Institute for Bioanalytical Chemistry, University of Kansas, Lawrence, KS, 66045, USA

    Craig Lunte

Authors
  1. Senem Şanli
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  2. Nurullah Şanli
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  3. Sibel A. Ozkan
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  4. Craig Lunte
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Corresponding author

Correspondence to Sibel A. Ozkan.

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The authors declare that they have no conflicts of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

Additional information

This paper is dedicated to the memory of our wonderful colleague, Dr. Craig Lunte, who recently passed away.

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Şanli, S., Şanli, N., Ozkan, S.A. et al. Development and Validation of a Green Capillary Electrophoretic Method for Determination of Polyphenolic Compounds in Red Wine Samples. Chromatographia 79, 1351–1358 (2016). https://doi.org/10.1007/s10337-016-3147-4

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  • DOI: https://doi.org/10.1007/s10337-016-3147-4

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