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Applications of Poly(Ethylene)Glycol (PEG) in Separation Science

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Abstract

The wide range of applications of poly(ethylene)glycol (PEG) in primarily chromatography and other closely related analytical methods has been reviewed. PEG has been used as mobile phase modifier in capillary electrophoresis (CE) as well as ion exchange, size exclusion, and hydrophobic interaction liquid chromatography (LC) methods. Generally in the presence of PEG, LC retention of macromolecules is altered and stability of their structure is maintained. PEG was effective in CE as a permanent coating for fused silica capillaries to shield free silanol groups that can cause protein adsorption to the wall resulting in band broadening and low recovery of the separated proteins. In gas chromatography, PEG-based stationary phases were applied for separation of polar analytes. PEG could also serve as an extraction medium in solid phase microextraction and aqueous two phase systems. Selected analytical applications, primarily LC and CE, involving PEG to facilitate the determination of either small molecules or macromolecules such as proteins in their native form are discussed and representative figures provided.

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Authors and Affiliations

  1. Department of Pharmaceutical Analytical Chemistry, Tanta University, Tanta, 31111, Egypt

    Fotouh R. Mansour

  2. Department of Chemistry and Biochemistry, Miami University, 651 E. High Street, Oxford, OH, 45056, USA

    Fotouh R. Mansour, Ling Zhou & Neil D. Danielson

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  1. Fotouh R. Mansour
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  2. Ling Zhou
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Correspondence to Neil D. Danielson.

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Mansour, F.R., Zhou, L. & Danielson, N.D. Applications of Poly(Ethylene)Glycol (PEG) in Separation Science. Chromatographia 78, 1427–1442 (2015). https://doi.org/10.1007/s10337-015-2983-y

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