Abstract
The bay scallop, Argopecten irradians irradians, introduced from North America, has become one of the most important aquaculture species in China. Inan effort to identify scallop genes involved in host defense, a high-quality cDNA library was constructed from whole body tissues of the bay scallop. A total of 5828 successful sequencing reactions yielded 4995 expressed sequence tags (ESTs) longer than 100 bp. Cluster and assembly analyses of the ESTs identified 637 contigs (consisting of 2853 sequences) and 2142 singletons, totaling 2779 unique sequences. Basic Local Alignment Search Tool (BLAST) analysis showed that the majority (73%) of the unique sequences had no significant homology (E-value ≤ 0.005) to sequences in GenBank. Among the 748 sequences with significant GenBank matches, 160 (21.4%) were for genes related to metabolism, 131 (17.5%) for cell/organism defense, 124 (16.6%) for gene/protein expression, 83 (11.1%) for cell structure/motility, 70 (9.4%) for cell signaling/communication, 17 (2.3%) for cell division, and 163 (21.8%) matched to genes of unknown functions. The list of host-defense genes included many genes with known and important roles in innate defense such as lectins, defensins, proteases, protease inhibitors, heat shock proteins, antioxidants, and Toll-like receptors. The study provides a significant number of ESTs for gene discovery and candidate genes for studying host defense in scallops and other molluscs.
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Acknowledgments
We thank Dr. Zhonghua Cai, Dr. Lingling Wang, Dr. Lihua Qiu, and Ms. Duojiao Ni for technical advice and helpful discussions. Sequencing of the scallop cDNA library was conducted at the Huada Genomic Center (Beijing, China). This study was supported by NSFC (Award 39825121 to X.G. and 40276045 to L.S.), 863 High Technology Project (No. 2002AA626020 to L.S.), and 973 national key fundamental research program (No. G1999012008 to L.S.). Guo's participation is supported by Rutgers University.
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Song, L., Xu, W., Li, C. et al. Development of Expressed Sequence Tags from the Bay Scallop, Argopecten irradians irradians. Mar Biotechnol 8, 161–169 (2006). https://doi.org/10.1007/s10126-005-0126-4
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DOI: https://doi.org/10.1007/s10126-005-0126-4