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Guanidinoamidized linear polyethyleneimine for gene delivery

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Abstract

Guanidine was introduced to low molecular weight linear polyethyleneimine (LPEI) via amide groups, to explore the effect of both guanidine degree and pendant chain length on its transfection behavior. The resulting guanidinoamidized LPEIs (GLPEIs) could dramatically reduce LPEI’s toxicity, enhance its DNA-packaging capability, cellular uptake and therefore transfection efficiency. These polyplexes were taken up very efficiently via caveolae-mediated endocytosis and their transfection efficiencies in ovarian cancer cells were significantly improved compared to native LPEI10k polyplexes. Among these GLPEIs, LPEI-C3-G100 showed higher DNA affinity even than LPEI25k and the highest transfection efficiency, probably due to the optimization of polymer chain flexibility. Of notice, LPEI-C3-G100 polyplexes could more effectively accumulate into cytoplasm than LPEI25k, although the transfection efficiency of LPEI-C3-G100 polyplexes was not superior to that of LPEI25k polyplexes, which would be probably attributed to the more efficient release of LPEI25k polyplexes than LPEI-C3-G100 polyplexes in the cytoplasm.

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Author information

Authors and Affiliations

  1. Institute for Individualized Medicine, Hangzhou First People’s Hospital, Hangzhou, 310006, China

    Bo Zhang & Neng-ming Lin  (林能明)

  2. Center for Bionanoengineering and the State Key Laboratory for Chemical Engineering, Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, China

    Mei-hua Sui & You-qing Shen  (申有青)

  3. Laboratory of Clinical Pharmacy, Zhejiang Cancer Hospital, Hangzhou, 310022, China

    Bo Zhang

  4. Department of Chemical and Petroleum Engineering, University of Wyoming, Laramie, Wyoming, 82071, USA

    Bo Zhang, Xin-peng Ma & Maciej Radosz

  5. Department of Animal Science, University of Wyoming, Laramie, Wyoming, 82071, USA

    Edward Van Kirk & William J. Murdoch

Authors
  1. Bo Zhang
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  2. Xin-peng Ma
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  3. Mei-hua Sui
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  4. Edward Van Kirk
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  5. William J. Murdoch
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  6. Maciej Radosz
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  7. Neng-ming Lin  (林能明)
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  8. You-qing Shen  (申有青)
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Corresponding authors

Correspondence to Neng-ming Lin  (林能明) or You-qing Shen  (申有青).

Additional information

This work was financially supported by the Qianjiang Talent Program of Zhejiang Province (2014.1–2015.12, Zhang Bo), the National Natural Science Fund for Distinguished Young Scholars (No. 50888001), the National Natural Science Foundation of China (No. 20974096), Zhejiang Provicial Program for the Cultivation of High-level Innovative Health Talents (2010-190-4), and the U.S. National Science Foundation (No. CBET 0753109, DMR-0705298) and Department of Defense (No. BC083821).

These two authors are joint first authors.

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Zhang, B., Ma, Xp., Sui, Mh. et al. Guanidinoamidized linear polyethyleneimine for gene delivery. Chin J Polym Sci 33, 908–919 (2015). https://doi.org/10.1007/s10118-015-1644-9

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  • DOI: https://doi.org/10.1007/s10118-015-1644-9

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