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Simple action potential measurement of cardiac cell sheet utilizing electronic sheet

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Abstract

In this study, a device for measuring the action potential of cardiac cell sheets was developed. The action potential was measured using a device comprising a 2-µm-thick parylene film with a silver electrode printed on it, which was referred to as the “electronic sheet.” The thin parylene film exhibits high biocompatibility and flexibility. Therefore, it demonstrates promise for biomedical microelectromechanical system applications. In this study, a cell sheet was used because the interest it had garnered in regenerative medicine for creating cardiac tissue in vitro similar to that in vivo. A high-efficiency drug development system can be realized by combining cell sheet technology and fabricating a flexible electronic sheet. The action potential of a cardiac cell sheet from a rat was measured using the as-developed flexible electronic sheet.

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References

  1. Cabinet Office, Government of Japan, Sect. 1 The situation of aging. http://www8.cao.go.jp/kourei/whitepaper/w-2016/html/gaiyou/s1_1.html. Accessed 26 Sep 2017

  2. Scott CW et al (2013) Human induced pluripotent stem cells and their use in drug discovery for toxicity testing. Toxicol Lett 219:49–58

    Article  Google Scholar 

  3. Thomson JA et al (1998) Embryonic stem cell lines derived from human blastocysts. Science 282:1145–1148

    Article  Google Scholar 

  4. Takahashi K et al (2007) Introduction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 31:861–872. https://doi.org/10.1016/j.cell.2007.11.019

    Article  Google Scholar 

  5. Arai T et al (2016) Fabrication of micro— gelatin fiber utilizing coacervation method. Artif Life Robot 22:197–202. https://doi.org/10.1007/s10015-016-0344-z

    Article  Google Scholar 

  6. Ryu-ichiro T et al (2017) Fundamental characteristics of printed gelatin utilizing micro 3D printer. Artif Life Robot 22:316–320. https://doi.org/10.1007/s10015-016-0348-8

    Article  Google Scholar 

  7. Umezu S (2012) BioCell print utilizing patterning with electrostatically injected droplet (PELID) method. Artif Life Robot 17:1–4. https://doi.org/10.1007/s10015-012-0018-4

    Article  Google Scholar 

  8. Shimizu T (2014) Cell sheet-cod tissue engineering for fabricating 3-dimensional heart tissues. Circ J 78:2594–2603

    Article  Google Scholar 

  9. Sakaguchi K et al (2013) ser engineering of vascularized tissue surrogates. Sci Rep 3:1316

    Article  Google Scholar 

  10. Sekine H et al (2013) In vitro fabrication of functional three-dimensional tissues with perfusable blood vessels. Nat Commun 4:1399

    Article  Google Scholar 

  11. Sekitani T et al (2016) Ultraflexible organic amplifier with biocompatible gel electrodes. Nat Commun 7:11425

    Article  Google Scholar 

  12. Kim D et al (2012) With capabilities in cardiac electrophysiological mapping and ablation therapy. Nat Mater 10:316–323

    Article  Google Scholar 

  13. Constantinescu G et al (2015) Epidermal electronics for electromyography: an application to swallowing therapy. Med Eng Phys 38:807–812

    Article  Google Scholar 

  14. Ohya T et al (2017) Simple action potential measurement of cardiac cell sheet utilizing electronic sheet. In: Proceedings of AROB 22nd, pp 933–936

  15. Shimizu T et al (2003) Cell sheet engineering for myocardial tissue reconstruction. Biomaterials 24:2309–2316

    Article  Google Scholar 

  16. Kim BJ et al (2016) Micromachining of Parylene C for bioMEMS. Polym Adv Technol 27:564–576

    Article  Google Scholar 

  17. Castagnola V et al (2015) Parylene-based flexible neural probes with PEDOT coated surface for brain stimulation and recording Biosens Bioelectron 67:450–457

    Article  Google Scholar 

  18. Fukuda K et al (2015) Reverse-offset printing optimized for scalable organic thin-film transistors with submicrometer channel lengths. Adv Electron Mater 1(8). https://doi.org/10.1002/aelm.201500145

  19. Meng E et al (2015) Plasma removal of parylene c. J Micromech Microeng 18:1–13

    Google Scholar 

  20. Haraguchi Y et al (2012) Fabrication of functional three-dimensional tissues by stacking cell sheets in vitro. Nat Protoc 7:850–858

    Article  Google Scholar 

  21. Takehara H et al (2015) Controlling shape and position of vascular formation in engineered tissues by arbitrary assembly of endothelial cells. Biofabrication 7:45006

    Article  Google Scholar 

  22. Ngo TX et al (2013) Endothelial cell behavior inside myoblast sheets with different thickness. Biotechnol Lett 35:1001–1008

    Article  Google Scholar 

Download references

Acknowledgements

T. Shimizu is a member of Scientific Advisory Board of CellSeed Inc. and is a shareholder of CellSeed Inc. CellSeed Inc. has licenses for certain cell sheet-related technologies and patents from Tokyo Women’s Medical University. Tokyo Women’s Medical University received research funds from CellSeed Inc. This work was supported by JSPS Kakenhi Grant number 16H04308 and 16K14203.

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Authors and Affiliations

  1. Graduate School of Creative Science and Engineering, Waseda University, Tokyo, Japan

    Takashi Ohya & Kazuki Nakazono

  2. Institute of Advanced Biomedical Engineering and Science, TWIns, Tokyo Women’s Medical University, Tokyo, Japan

    Takashi Ohya, Kazuki Nakazono, Tetsutaro Kikuchi, Daisuke Sasaki & Tatsuya Shimizu

  3. Faculty of Science and Engineering, TWIns, Waseda University, Tokyo, Japan

    Katsuhisa Sakaguchi

  4. RIKEN Thins-Film Device Laboratory and Center for Emergent Mater Science, Saitama, Japan

    Kenjiro Fukuda & Takao Someya

  5. JST PRESTO, Saitama, Japan

    Kenjiro Fukuda

  6. Undergraduate School of Creative Science and Engineering, Waseda University, Tokyo, Japan

    Shinjiro Umezu

Authors
  1. Takashi Ohya
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  2. Kazuki Nakazono
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  3. Tetsutaro Kikuchi
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  4. Daisuke Sasaki
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  5. Katsuhisa Sakaguchi
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  6. Tatsuya Shimizu
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  7. Kenjiro Fukuda
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  8. Takao Someya
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  9. Shinjiro Umezu
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Corresponding author

Correspondence to Shinjiro Umezu.

Additional information

This work was presented in part at the 2nd International Symposium on BioComplexity, Beppu, Oita, January 19–21, 2017.

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Ohya, T., Nakazono, K., Kikuchi, T. et al. Simple action potential measurement of cardiac cell sheet utilizing electronic sheet. Artif Life Robotics 23, 321–327 (2018). https://doi.org/10.1007/s10015-018-0429-y

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  • DOI: https://doi.org/10.1007/s10015-018-0429-y

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