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Diversity of Actinobacterial community in saline sediments from Yunnan and Xinjiang, China

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Abstract

The diversity and community structures of actinobacteria in saline sediments collected from Yunnan and Xinjiang Provinces, China, were investigated with cultivation and 16S rRNA gene analysis. A total of 163 actinobacterial isolates were obtained, and they were affiliated with the order Actinomycetales (distributed into five suborders: Streptosporangineae, Micrococcineae, Streptomycineae, Pseudonocardineae, and Glycomycineae). A total of 748 actinobacterial 16S rRNA gene clones were examined, and they could be classified into Actinomycetales, Acidimicrobiales, and unclassified actinobacteria. The Actinomycetales sequences were distributed into nine suborders: Streptosporangineae, Glycomycineae, Micromonosporineae, Pseudonocardineae, Corynebacterineae, Frankineae, Propionibacterineae, Streptomycineae, and Micrococcineae. The unclassified actinobacteria contained three new clusters at the level of subclass or order. Our 16S rRNA gene phylogenetic data indicated that actinobacterial communities were very diverse in the investigated saline sediments (salinity 0.4–11.6%) and some actinobacterial members may be halotolerant or halophilic. The actinobacterial community structures in the saline sediments were different from those in marine and freshwater environments. Our data have implications for a better understanding of the distribution of Actinobacteria in saline environments.

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Acknowledgments

This research was supported by the National Basic Research Program of China (No. 2004CB719601), the Key Project of Chinese Ministry of Education (No. 206139), the National Natural Science Foundation of China (No. 30600001, 30660005, 30860002, 30870005), and the Yunnan Provincial Natural Science Foundation (project no. 2007C167 M). W.-J. Li was supported by Program for New Century Excellent Talents in Yunnan University. We are grateful to two anonymous reviewers for their constructive comments that greatly improved the quality of the manuscript.

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Authors and Affiliations

  1. The Key Laboratory for Microbial Resources of the Ministry of Education, Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, China

    Jinyuan Wu, Xiaoyang Zhi, Shukun Tang & Wenjun Li

  2. Key Laboratory of Protection and Utilization of Biological Resources, Tarim Basin of Xinjiang Production and Construction Corps, Tarim University, Alar, Xinjiang, 843300, China

    Tongwei Guan & Lili Zhang

  3. Geomicrobiology Laboratory, State Key Laboratory of Geological Processes and Mineral Resources, School of Earth Sciences and Resources, China University of Geosciences, Beijing, 100083, China

    Hongchen Jiang & Hailiang Dong

  4. Department of Geology, Miami University, Oxford, OH, 45056, USA

    Hailiang Dong

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  1. Jinyuan Wu
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  4. Xiaoyang Zhi
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  7. Lili Zhang
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  8. Wenjun Li
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Correspondence to Wenjun Li.

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Communicated by F. Robb.

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Wu, J., Guan, T., Jiang, H. et al. Diversity of Actinobacterial community in saline sediments from Yunnan and Xinjiang, China. Extremophiles 13, 623–632 (2009). https://doi.org/10.1007/s00792-009-0245-3

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  • DOI: https://doi.org/10.1007/s00792-009-0245-3

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