Summary.
Providence Virus (PrV) is a non-envoloped, T = 4 icosahedral beta-tetravirus that undergoes autocatalytic cleavage of its coat protein precursor after capsid assembly. This is also a well characterized function of Nudaurelia capensis ω virus (NωV), a member of the related omegatetraviruses, whose x-ray structure has been determined. Virus-like particle (VLP) production of PrV in a recombinant baculovirus expression system was attempted to obtain high VLP yields for comparison of structural and autocatalytic active site properties between these virus groups. This resulted in insoluble aggregates of PrV coat protein even though NωV VLPs have been successfully produced in the same system. Betatetraviruses may be more dependent on compartmentalization and availability of their full-length genome for proper folding and assembly. However, crystals were grown of limited quantities of authentic PrV produced in cell culture and a partial X-ray data set collected to 3.8 Å resolution. The virus particle position and orientation in the unit cell was determined by space group consideration and rotation function analysis. A phasing model, based on NωV, was developed to initiate the structure solution of PrV.
Article PDF
Similar content being viewed by others
References
D Agrawal J Johnson (1992) ArticleTitleSequence and analysis of the capsid protein of nudaurelia capensis ω virus, an insect virus with T = 4 icosahedral symmetry. Virology 190 806–814 Occurrence Handle1519360 Occurrence Handle10.1016/0042-6822(92)90918-F Occurrence Handle1:CAS:528:DyaK3sXks1GrtL8%3D
D Agrawal J Johnson (1995) ArticleTitleAssembly of the T = 4 Nudaurelia capensis omega virus capsid protein, post translational cleavage, and specific encapsidation of its mRNA in a baculovirus expression system. Virology 207 89–97 Occurrence Handle7871755 Occurrence Handle10.1006/viro.1995.1054 Occurrence Handle1:CAS:528:DyaK2MXjslCmsbc%3D
MA Canady M Tihova TN Hanzlik JE Johnson M Yeager (2000) ArticleTitleLarge conformational changes in the maturation of a simple RNA virus, Nudaurelia capensis ω virus (NωV). J Mol Biol 299 573–584 Occurrence Handle10835268 Occurrence Handle10.1006/jmbi.2000.3723 Occurrence Handle1:CAS:528:DC%2BD3cXjs12nsLg%3D
AJ Fisher JE Johnson (1993) ArticleTitleOrdered duplex RNA controls capsid architecture in an icosahedral animal virus. Nature 361 176–179 Occurrence Handle8421524 Occurrence Handle10.1038/361176a0 Occurrence Handle1:CAS:528:DyaK3sXns1Omtw%3D%3D
KH Gordon MR Williams DA Hendry TN Hanzlik (1999) ArticleTitleSequence of the genomic RNA of nudaurelia beta virus (Tetraviridae) defines a novel virus genome organization. Virology 258 42–53 Occurrence Handle10329566 Occurrence Handle10.1006/viro.1999.9677 Occurrence Handle1:CAS:528:DyaK1MXivFOqtr0%3D
TN Hanzlik SJ Dorrian KH Gordon PD Christian (1993) ArticleTitleA novel small RNA virus isolated from the cotton bollworm, Helicoverpa armigera. J Gen Virol 74 1805–1810 Occurrence Handle8376959 Occurrence Handle1:CAS:528:DyaK3sXmsVGisLo%3D Occurrence Handle10.1099/0022-1317-74-9-1805
TN Hanzlik KH Gordon (1997) ArticleTitleThe Tetraviridae. Adv Virus Res 48 101–168 Occurrence Handle9233432 Occurrence Handle1:CAS:528:DyaK2sXntVeju7c%3D
Johnson JE, Munshi S, Liljas L, Agrawal D, Olson NH, Reddy V, Fisher A, McKinney B, Schmidt T, Baker TS (1994) Comparative studies of T = 3 and T = 4 icosahedral RNA insect viruses. Arch Virol [Suppl 9]: 497–512
DJ Miller MD Schwartz P Ahlquist (2001) ArticleTitleFlock house virus RNA replicates on outer mitochondrial membranes in Drosophila cells. J Virol 75 11664–11676 Occurrence Handle11689648 Occurrence Handle1:CAS:528:DC%2BD3MXotlaqs7o%3D
S Munshi L Liljas J Cavarelli W Bomu B McKinney V Reddy JE Johnson (1996) ArticleTitleThe 2.8 Å structure of a T = 4 animal virus and its implications for membrane translocation of RNA. J Mol Biol 261 1–10 Occurrence Handle8760498 Occurrence Handle10.1006/jmbi.1996.0437 Occurrence Handle1:CAS:528:DyaK28XkvFOgtr8%3D
NH Olson TS Baker JE Johnson DA Hendry (1990) ArticleTitleThe three-dimensional structure of frozen-hydrated Nudaurelia capensis beta virus, a T = 4 insect virus. J Struct Biol 105 111–122 Occurrence Handle1712620 Occurrence Handle10.1016/1047-8477(90)90105-L Occurrence Handle1:STN:280:By6B1MbktlM%3D
Otwinowski Z, Minor W (1997) Processing of X-ray diffraction data collected in oscillation mode. In: Carter CW Jr, Sweet RM (eds) Macromolecular crystallography, part A. Academic Press Methods in Enzymology vol 276, 307–326
FM Pringle KH Gordon TN Hanzlik J Kalmakoff PD Scotti VK Ward (1999) ArticleTitleA novel capsid expression strategy for Thosea asigna virus (Tetraviridae). J Gen Virol 80 1855–1863 Occurrence Handle10423156 Occurrence Handle1:CAS:528:DyaK1MXksFOlu7Y%3D
FM Pringle J Kalmakoff VK Ward (2001) ArticleTitleAnalysis of the capsid processing strategy of Thosea asigna virus using baculovirus expression of virus-like particles. J Gen Virol 82 259–266 Occurrence Handle11125178 Occurrence Handle1:CAS:528:DC%2BD3MXjtVGntg%3D%3D
FM Pringle KN Johnson CL Goodman AH McIntosh LA Ball (2003) ArticleTitleProvidence virus: a new member of the Tetraviridae that infects cultured insect cells. Virology 306 359–370 Occurrence Handle12642108 Occurrence Handle10.1016/S0042-6822(02)00052-1 Occurrence Handle1:CAS:528:DC%2BD3sXitVKmt7Y%3D
M Restrepo-Hartwig P Ahlquist (1999) ArticleTitleBrome mosaic virus RNA replication proteins 1a and 2a colocalize and 1a independently localizes on the yeast endoplasmic reticulum. J Virol 73 10303–10309 Occurrence Handle10559348 Occurrence Handle1:CAS:528:DyaK1MXnsV2mu7g%3D
MG Rossmann (1990) ArticleTitleThe molecular replacement method. Acta Crystallogr A 46 IssueIDPt 2 73–82 Occurrence Handle2180438
L Tang KN Johnson LA Ball T Lin M Yeager JE Johnson (2001) ArticleTitleThe structure of pariacoto virus reveals a dodecahedral cage of duplex RNA. Nat Struct Biol 8 77–83 Occurrence Handle11135676 Occurrence Handle1:CAS:528:DC%2BD3MXjslyqsg%3D%3D
DJ Taylor NK Krishna MA Canady A Schneemann JE Johnson (2002) ArticleTitleLarge-scale, pH-dependent, quaternary structure changes in an RNA virus capsid are reversible in the absence of subunit autoproteolysis. J Virol 76 9972–9980 Occurrence Handle12208973 Occurrence Handle10.1128/JVI.76.19.9972-9980.2002 Occurrence Handle1:CAS:528:DC%2BD38XntFGksbk%3D
DJ Taylor JE Johnson (2005) ArticleTitleFolding and particle assembly are disrupted by single point mutations near the autocatalytic site of Nudaurelia capensis ω virus capsid protein. Protein Sci 14 401–408 Occurrence Handle15659373 Occurrence Handle10.1110/ps.041054605 Occurrence Handle1:CAS:528:DC%2BD2MXhtFOrsLs%3D
L Tong MG Rossmann (1997) ArticleTitleRotation function calculations with GLRF program. Methods Enzymol 276 594–611 Occurrence Handle9048382 Occurrence Handle1:CAS:528:DyaK2sXivFehtrg%3D
LA Tong MG Rossmann (1990) ArticleTitleThe locked rotation function. Acta Crystallogr A 46 783–792 Occurrence Handle2174243 Occurrence Handle10.1107/S0108767390005530
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Taylor, D., Speir, J., Reddy, V. et al. Preliminary x-ray characterization of authentic providence virus and attempts to express its coat protein gene in recombinant baculovirus. Arch Virol 151, 155–165 (2006). https://doi.org/10.1007/s00705-005-0637-3
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00705-005-0637-3