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Physical localization of rRNA genes by fluorescence in situ hybridization (FISH) and analysis of spacer length variants of 45S rRNA (slvs) genes in some species of genus Sesbania

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Abstract

The somatic chromosome numbers 2n = 12 in S. macrantha, S. coerulescens and 2n = 24 in S. simplicuscula were determined, additionally the contradictory chromosome numbers of S. bispinosa (2n = 12, 13, 14, 24) and S. pachycarpa (2n 12, 14) were determined as 2n = 24 and 2n = 14, respectively. The number of 5S rDNA sites in chromosome pair 1 was highly conserved in all the diploid and tetraploid species studied irrespective of their geographic distribution, suggesting that all diploid and tetraploid species/cytotypes of Sesbania analyzed in the present study are in close proximity. Cytogenetic mapping of the 45S multi-gene family was also carried out using fluorescence in situ hybridization. 45S rDNA was consistently located on short or long arms of two sub-metacentric chromosome pairs except on one chromosome pair in S. macrantha and on three chromosome pairs in S. bispinosa and S. cannabina. Out of these nine species, we observed the homogenization of intergenic spacer in six species and find only one spacer length variant (slv) located on one to three chromosome loci. However, three of the species were observed to have two slvs located on two different chromosomes. The species were grouped as per their evolutionary relationship on the basis of the results of the present study.

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Acknowledgments

The authors are thankful to Dr. P. Zhang, W. J. Raupp, D. L. Wilson, Dr. H. S. Balyan for their valuable suggestions and help at the beginning and during the experimental work. This work was supported by a special USDA-CSREES grant to the Wheat Genetic and Genomic Resources Center at Kansas State University. One of the authors (S.K.) is grateful to the Department of Science and Technology and Department of Biotechnology, Ministry of Science and Technology, Government of India for financial support. This is contribution number 13-219-J from the Kansas Agricultural Experiment Station, Kansas State University.

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Authors and Affiliations

  1. Molecular Cytogenetics Laboratory, Department of Molecular Biology and Genetic Engineering, College of basic Sciences and Humanities, G. B. Pant University of Agriculture and Technology, U. S. Nagar, 263145, Pantnagar, Uttarakhand, India

    Sundip Kumar

  2. Wheat Genetic and Genomic Resources Center, Department of Plant Pathology, Throckmorton Plant Sciences Center, Kansas State University, Manhattan, KS, 66506-5502, USA

    Bernd Friebe & Bikram S. Gill

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  1. Sundip Kumar
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  2. Bernd Friebe
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  3. Bikram S. Gill
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Correspondence to Sundip Kumar.

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Kumar, S., Friebe, B. & Gill, B.S. Physical localization of rRNA genes by fluorescence in situ hybridization (FISH) and analysis of spacer length variants of 45S rRNA (slvs) genes in some species of genus Sesbania . Plant Syst Evol 300, 1793–1802 (2014). https://doi.org/10.1007/s00606-014-1006-z

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  • DOI: https://doi.org/10.1007/s00606-014-1006-z

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