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Electrocatalytic oxidation of NADH at mesoporous carbon modified electrodes

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Abstract

The electrochemical oxidation of β-nicotinamine adenine dinucleotide (NADH) was investigated at a glassy carbon electrode modified with carbon mesoporous materials (CMM). Due to the large surface area and electro-catalytic properties of CMM, the overpotential of the electrodes toward the oxidation of NADH is decreased by 595 mV in aqueous solution at neutral pH. The anodic peak currents increase steadily with the concentration of NADH in the range from 2 µM to 1.1 mM, the detection limit being 1.0 µM at pH 7.2 and a potential of +0.3 V vs. SCE. The apparent Michaelis-Menten constant is ∼21.5 μM. The results enable NADH to be sensed at a low potential and are promising with respect to the design of dehydrogenase-based amperometric biosensors.

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Acknowledgements

This work was supported by the NSFC 20775016, Shanghai Leading Academic Discipline Project B108, B109 and Shuguang Project 06SG02.

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Authors and Affiliations

  1. Department of Chemistry, Institute of Biomedical Sciences and Institute of Advanced Materials, Fudan University, Shanghai, 200433, People’s Republic of China

    Ying Wang, Chunping You, Song Zhang, Jilie Kong, Dongyuan Zhao & Baohong Liu

  2. BIOMEM, University of Perpignan Via Domitia, 66860, Perpignan, France

    Jean-Louis Marty

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  1. Ying Wang
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  2. Chunping You
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  3. Song Zhang
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  4. Jilie Kong
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  5. Jean-Louis Marty
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  6. Dongyuan Zhao
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  7. Baohong Liu
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Correspondence to Dongyuan Zhao or Baohong Liu.

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Wang, Y., You, C., Zhang, S. et al. Electrocatalytic oxidation of NADH at mesoporous carbon modified electrodes. Microchim Acta 167, 75 (2009). https://doi.org/10.1007/s00604-009-0217-4

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  • DOI: https://doi.org/10.1007/s00604-009-0217-4

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