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Cell wall polysaccharides from cell suspension cultures of the Atlantic Forest tree Rudgea jasminoides (Rubiaceae)

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Abstract

Rudgea jasminoides (Rubiaceae) is a tropical tree species native of the Atlantic Forest in the south of Brazil. Previous studies with leaf cell walls of R. jasminoides showed a different proportion of cross-linked glycans compared to what is usually reported for eudicots. However, due to the difficulties of working with whole plant organs, cell suspensions of R. jasminoides, consisting of predominantly undifferentiated cells with mainly primary cell walls, were used to examine cell walls and extracellular soluble polysaccharides (EP) released into the culture medium. Sugar composition and linkage analysis showed homogalacturonans, xylogalacturonans and arabinogalactans to be the predominant EP. In the cell wall, homogalacturonans and arabinogalactans are the major pectins, and xyloglucans and xylans are the major cross-linking glycans. The presence of xylogalacturonans in the R. jasminoides cell cultures seems to be related to the occurrence of a homogeneous cell suspension with loosely attached cells. Although all alkali extractions from the cell walls yielded amounts of xyloglucan that exceed those of the xylans, the latter was found in a proportion that is higher than what has been usually reported for primary cell walls of most eudicots. The xyloglucan from cell walls of cell suspension cultures of R. jasminoides has low fucosylation levels and high proportion of galactosyl residues, a branching pattern commonly found in storage cell-wall xyloglucans.

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Acknowledgments

This work was financially supported by the Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP, 98/05124-8 and 05/04139-7). Thanks are also due to Conselho Nacional de Desenvolvimento Científico e Tecnológico - CNPq for researcher fellowships to M.S. Buckeridge and M.R. Braga and to FAPESP for a PhD fellowship to C.J.F. Oliveira Júnior. The authors thank Dr Kelly Simões for help with the figures.

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Authors and Affiliations

  1. Núcleo de Pesquisa em Plantas Ornamentais, Instituto de Botânica, São Paulo, SP, Brazil

    Clóvis J. F. Oliveira Júnior

  2. Universidade Federal de São Paulo, Campus Diadema, Diadema, SP, Brazil

    Aline A. Cavalari

  3. Department of Plant Pathology, Purdue University, West Lafayette, IN, USA

    Nicholas C. Carpita

  4. Departamento de Botânica, Universidade de São Paulo, São Paulo, SP, Brazil

    Marcos S. Buckeridge

  5. Núcleo de Pesquisa em Fisiologia e Bioquímica, Instituto de Botânica, C.P. 3005, São Paulo, SP, 01061-970, Brazil

    Marcia R. Braga

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  1. Clóvis J. F. Oliveira Júnior
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  2. Aline A. Cavalari
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  3. Nicholas C. Carpita
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  4. Marcos S. Buckeridge
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  5. Marcia R. Braga
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Correspondence to Marcia R. Braga.

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Communicated by R. D. Guy.

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Oliveira Júnior, C.J.F., Cavalari, A.A., Carpita, N.C. et al. Cell wall polysaccharides from cell suspension cultures of the Atlantic Forest tree Rudgea jasminoides (Rubiaceae). Trees 24, 713–722 (2010). https://doi.org/10.1007/s00468-010-0441-x

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