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Responses of mycorrhizal jack pine (Pinus banksiana) seedlings to NaCl and boron

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Abstract

In earlier studies, we established that mycorrhizal associations protect plants against salt stress. However, elevated boron levels are often present in saline soils and little is known about the effects of boron on salt resistance of mycorrhizal plants. In the present study, we inoculated jack pine (Pinus banksiana) seedlings with Hebeloma sp., Suillus tomentosus and Wilcoxina mikolae var. mikolae to study the effects of mycorrhizal associations on seedling responses to boron and salt. Seedlings were grown in the greenhouse and subjected to 60 mM NaCl, 2 mM H3BO3 or 60 mM NaCl + 2 mM H3BO3 treatments for 4 weeks. Dry weights, shoot:root ratios and chlorophyll concentrations were higher in inoculated seedlings for all treatments compared with the non-inoculated plants. When applied with NaCl, B aggravated needle necrosis while reducing Cl concentrations in shoots of non-inoculated plants. Plants treated with 2 mM H3BO3 + 60 mM NaCl had similar concentrations of Na and B to those that were treated separately with 60 mM NaCl and 2 mM H3BO3. Plants inoculated with mycorrhizal fungi had lower shoot Na concentrations compared with non-inoculated seedlings, but showed relatively little impact from elevated B concentrations.

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Acknowledgments

This study was funded through the Natural Sciences and Engineering Research Council of Canada Strategic Research Grant to JJZ and MDJ.

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Authors and Affiliations

  1. Department of Renewable Resources, University of Alberta, 442 Earth Sciences Bldg., Edmonton, AB, T6G 2E3, Canada

    Mónica Calvo Polanco & Janusz J. Zwiazek

  2. Biology Program, Biology and Physical Geography Unit, UBC Okanagan, 3333 University Way, Kelowna, BC, V1V 1V7, Canada

    Melanie D. Jones

  3. Edmonton Research Centre, Syncrude Canada Ltd., 9421 17 Avenue, Edmonton, AB, T6N 1H4, Canada

    Michael D. MacKinnon

Authors
  1. Mónica Calvo Polanco
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  2. Janusz J. Zwiazek
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  3. Melanie D. Jones
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  4. Michael D. MacKinnon
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Correspondence to Janusz J. Zwiazek.

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Communicated by T. Hogetsu.

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Calvo Polanco, M., Zwiazek, J.J., Jones, M.D. et al. Responses of mycorrhizal jack pine (Pinus banksiana) seedlings to NaCl and boron. Trees 22, 825–834 (2008). https://doi.org/10.1007/s00468-008-0243-6

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  • DOI: https://doi.org/10.1007/s00468-008-0243-6

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