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Reciprocal interactions of Fgf10/Fgfr2b modulate the mouse tongue epithelial differentiation

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Abstract

The molecular mechanisms for epithelial differentiation have been studied by observing skin development in embryogenesis, but the early signaling modulations involved in tongue epithelial differentiation are not completely understood. Based on the gene expression patterns of the Fgf signaling molecules and previous results from Fgf10 and Fgfr2b knockout mice, it was hypothesized that there would be fundamental signaling interactions through the epithelial Fgfr2b and its mesenchymal ligand Fgf10 to regulate tongue epithelium differentiation. To elucidate these reciprocal interactions in tongue epithelial differentiation, this study employed an in vitro tongue organ culture system with antisense-oligodeoxynucleotides (AS-ODNs) and recombinant protein-soaked bead implantation for the loss-of-function and gain-of-function studies. Functional analysis of Fgf signaling revealed precise reciprocal interactions, which showed that mesenchymal Fgf10 rather than Fgf7 modulates tongue epithelial differentiation via Fgfr2b in a temporal- and spatial-specific manner.

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Authors and Affiliations

  1. School of Life Science and Biotechnology, Kyungpook National University, Daegu, South Korea

    Wern-Joo Sohn, Sanggyu Lee & Zae Young Ryoo

  2. Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University, 101 Dongin-dong 2ga, Joong-gu, Daegu, 700–422, South Korea

    Hye-In Jung, Min-A Choi, Jin-Hyun Han, Gi-Jeong Gwon & Jae-Young Kim

  3. Department of Histology, Cytology and Developmental Anatomy, Nihon University School of Dentistry at Matsudo, Chiba, Japan

    Hitoshi Yamamoto

  4. Department of Oral Pathology and Regenerative Medicine, School of Dentistry, IHBR, Kyungpook National University, Daegu, South Korea

    Eui-Kyun Park & Hong-In Shin

  5. Division in Anatomy and Developmental Biology, Department of Oral Biology, Research Center for Orofacial Hard Tissue Regeneration, Brain Korea 21 project, Oral Science Research Center, College of Dentistry, Yonsei Center of Biotechnology, Yonsei University, Seoul, South Korea

    Han-Sung Jung

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  1. Wern-Joo Sohn
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  2. Hye-In Jung
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  3. Min-A Choi
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Corresponding author

Correspondence to Jae-Young Kim.

Additional information

Wern-Joo Sohn and Hye-In Jung: contributed equally to this work.

This work was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (R13-2008-009-01002-0)", and by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD)” (KRF-2007-E00124).

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Sohn, WJ., Jung, HI., Choi, MA. et al. Reciprocal interactions of Fgf10/Fgfr2b modulate the mouse tongue epithelial differentiation. Cell Tissue Res 345, 265–273 (2011). https://doi.org/10.1007/s00441-011-1204-8

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  • DOI: https://doi.org/10.1007/s00441-011-1204-8

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