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Suppression subtractive hybridization and comparative expression of a pore-forming toxin and glycosyl hydrolase genes in Rhizoctonia solani during potato sprout infection

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Abstract

Rhizoctonia solani is a plant pathogenic fungus that causes black scurf on tubers and stem and stolon canker on underground parts of potato plant. Early in the season, the fungus attacks germinating sprouts underground before they emerge from the soil. Damage at this stage results in delayed emergence of weakened plants with poor and uneven stands. The mechanism underlying this phenomenon has been investigated in this study by coupling a cDNA-suppression subtractive hybridization (SSH) library to differential screening to identify transcripts of R. solani that are down-regulated during infection of potato sprouts. We report on the identification of 33 unique genes with functions related to carbohydrate binding, vitamin synthesis, pathogenicity, translation, ATP and nucleic acid binding and other categories. RACE-PCR was used to clone and characterize the first full-length cDNA clones, RSENDO1 and RSGLYC1 that encode for an eukaryotic delta-endotoxin CytB protein and an intracellular glycosyl hydrolase, respectively. Quantitative real-time PCR revealed the down-regulation of RSENDO1 during infection of potato sprouts and the up-regulation of RSGLYC1 when the fungus was grown on a cellulose-based nutrient medium. In contrast, additional experiments have highlighted the down-regulation of RSENDO1 when R. solani was co-cultured with the mycoparasite Stachybotrys elegans and the bacterial antagonist Bacillus subtilis B26. These results advance our understanding of R. solani–potato interaction in subterranean parts of the plant. Such approaches could be considered in building an efficient integrated potato disease management program.

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Acknowledgments

The authors thank D. Rivard for cDNA libraries construction. This work was financed by the Natural Sciences and Engineering Research Council of Canada (NSERC-Discovery) granted to S. Jabaji (Grant Number: 137135-13).

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Plant Science, Faculty of Agricultural and Environmental Sciences, McGill University, 21,111 Lakeshore Rd., Ste. Anne de Bellevue, Montreal, QC, H9X 3V9, Canada

    Rony Chamoun, Jamil Samsatly & Suha Jabaji

  2. Department of Infectious Disease, J. Craig Venter Institute, Rockville, MD, USA

    Suman B. Pakala

  3. Institute of Bioinformatics, University of Georgia, Athens, GA, USA

    Suman B. Pakala

  4. Department of Plant Pathology, Center for Integrated Fungal Research, North Carolina State University, Box 7616, Raleigh, NC, 27695-7616, USA

    Marc A. Cubeta

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  1. Rony Chamoun
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Correspondence to Suha Jabaji.

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Chamoun, R., Samsatly, J., Pakala, S.B. et al. Suppression subtractive hybridization and comparative expression of a pore-forming toxin and glycosyl hydrolase genes in Rhizoctonia solani during potato sprout infection. Mol Genet Genomics 290, 877–900 (2015). https://doi.org/10.1007/s00438-014-0962-x

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