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Evaluation of immunomagnetic separation method for detection of Giardia for different reaction times and reaction volumes

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Abstract.

Immunomagnetic separation (IMS) has been specified as a standard method for the measurement of Giardia. In this study, Dynal IMS was evaluated on the basis of recovery efficiencies of Giardia cysts for various IMS operational conditions. The average recoveries for Giardia in deionized, treated and raw water samples were 82.6±12.2% (n=6), 75.6±15.2% (n=3), and 70.6±18.2% (n=3), respectively. Significant changes in recovery were observed by altering the debris ratio and the debris components of water samples. Changing the reaction volume within the same vessel had no significant effect on cyst recovery efficiencies. However, prolonging the reaction time did increase recovery efficiencies.

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Authors and Affiliations

  1. Institute of Environmental Engineering, National Chiao Tung University, Hsinchu, Taiwan, R.O.C., , , , ,

    Bing-Mu Hsu, Chihpin Huang & Ying-Chien Chung

  2. Chiayi Christian Hospital, 539 Chung-Shiao Street, Chiayi, Taiwan, R.O.C., , , , ,

    Yu-Che Lai

  3. Department of Safety, Health and Environmental Engineering, National Kaohsiung First University of Science and Technology, 2 Juoyue Road, Nantsu, Kaohsiung, Taiwan, R.O.C., , , , ,

    Hua-Shan Tai

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  1. Bing-Mu Hsu
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  2. Chihpin Huang
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  3. Yu-Che Lai
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  4. Hua-Shan Tai
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  5. Ying-Chien Chung
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Hsu, BM., Huang, C., Lai, YC. et al. Evaluation of immunomagnetic separation method for detection of Giardia for different reaction times and reaction volumes. Parasitol Res 87, 472–474 (2001). https://doi.org/10.1007/s004360100377

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  • DOI: https://doi.org/10.1007/s004360100377

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