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Nitric oxide donor-mediated inhibition of phosphorylation shows that light-mediated degradation of photosystem II D1 protein and phosphorylation are not tightly linked

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Abstract

An outcome of the photochemistry during oxygenic photosynthesis is the rapid turn over of the D1 protein in the light compared to the other proteins of the photosystem II (PS II) reaction center. D1 is a major factor of PS II instability and its replacement a primary event of the PS II repair cycle. D1 also undergoes redox-dependent phosphorylation prior to its degradation. Although it has been suggested that phosphorylation modulates D1 metabolism, reversible D1 phosphorylation was reported not to be essential for PS II repair in Arabidopsis. Thus, the involvement of phosphorylation in D1 degradation is controversial. We show here that nitric oxide donors inhibit in vivo phosphorylation of the D1 protein in Spirodela without inhibiting degradation of the protein. Thus, D1 phosphorylation is not tightly linked to D1 degradation in the intact plant.

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Fig. 1
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Abbreviations

CAM:

Crassulacean acid metabolism

NaF:

Sodium fluoride

NO:

Nitric oxide

PS II:

Photosystem II

SIN-1:

3-Morpholinosydnonimine

SNOC:

S-Nitrosocysteine

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Acknowledgments

Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture.

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Authors and Affiliations

  1. Sustainable Agricultural Systems Laboratory, Building 001, Beltsville Agricultural Research Center, Agricultural Research Service, United States Department of Agriculture, Beltsville, MD, 20705-2350, USA

    Isabelle S. Booij-James & Autar K. Mattoo

  2. Department of Plant Sciences, Weizmann Institute of Science, 76100, Rehovot, Israel

    Marvin Edelman

Authors
  1. Isabelle S. Booij-James
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  2. Marvin Edelman
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  3. Autar K. Mattoo
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Correspondence to Autar K. Mattoo.

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Booij-James, I.S., Edelman, M. & Mattoo, A.K. Nitric oxide donor-mediated inhibition of phosphorylation shows that light-mediated degradation of photosystem II D1 protein and phosphorylation are not tightly linked. Planta 229, 1347–1352 (2009). https://doi.org/10.1007/s00425-009-0914-6

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