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Laser microdissection and cryogenic nuclear magnetic resonance spectroscopy: an alliance for cell type-specific metabolite profiling

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An Erratum to this article was published on 14 November 2006

Abstract

Laser microdissection was used as a tool to harvest secretory cavities (SC) from leaves of Dilatris pillansii Barker (Haemodoraceae) and from leaves and flowers of herbarium specimens of Dilatris corymbosa Berg. and Dilatris viscosa L. Cryogenic 1H NMR spectroscopy and HPLC analysis of microdissected samples indicated specific accumulation of methoxyphenylphenalenones in the SC. The structures of two novel and a known natural product in the secretory tissue were confirmed by comparison with authentic compounds isolated from rhizomes and roots from which further phenylphenalenones and phenylphenalenone glucosides were isolated and identified by spectroscopic methods. How it will be possible to use the LMD technique to localize natural products in specific plant cell populations is also discussed.

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Abbreviations

LMD:

Laser microdissection

NMR:

Nuclear magnetic resonance

SC:

Secretory cavities

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Acknowledgments

We wish to thank Tamara Krügel, Jena (Germany), for raising the plants from seed, D. Veit for constructing special slides for LMD and Emily Wheeler for proofreading. We thank A. Svatoš for recording mass spectra, A. Seeling for IR analysis, F. von Eggeling and Bettina Schimmel for the introduction to LMD, G. Turner, Pullman (USA) and Heike Heklau, Halle/S. (Germany), for helpful discussion about secretory cavities, H.-J. Zündorf and H. Manitz for discussions and providing herbarium material (Herbarium Haussknecht (JE), Friedrich Schiller University) and D. Heckel for comments on the manuscript.

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  1. Max-Planck-Institut für Chemische Ökologie, Beutenberg Campus, Hans-Knöll-Str. 8, 07745, Jena, Germany

    B. Schneider & D. Hölscher

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  1. B. Schneider
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  2. D. Hölscher
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Correspondence to B. Schneider.

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An erratum to this article can be found at http://dx.doi.org/10.1007/s00425-006-0434-6

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Schneider, B., Hölscher, D. Laser microdissection and cryogenic nuclear magnetic resonance spectroscopy: an alliance for cell type-specific metabolite profiling. Planta 225, 763–770 (2007). https://doi.org/10.1007/s00425-006-0404-z

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