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MUC16 in the lacrimal apparatus

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Abstract

The aim of the present study was to determine the possible expression of the mucin MUC16 in the lacrimal apparatus. Expression and distribution of MUC16 in lacrimal gland, accessory lacrimal glands, and nasolacrimal ducts was monitored by RT-PCR and immunohistochemistry. MUC16 was expressed and detected in all tissues investigated. Comparable to conjunctiva and cornea it was membrane-anchored in accessory lacrimal glands whereas in lacrimal gland acinar cells and columnar cells of the nasolacrimal ducts it was stored in intracytoplasmic vesicles without membrane-association. Subepithelial serous glands of the nasolacrimal ducts revealed staining of the secretion product. Intracelluar production of MUC16 is present in lacrimal gland and epithelial cells of the nasolacrimal ducts but it is not clear whether this MUC16 is secreted. MUC16 seems to be shedded or secreted from the epithelial surface of subepithelial serous glands of the nasolacrimal ducts. Our results show that MUC16 is present in the whole lacrimal apparatus. Its distribution pattern suggests different physiological functions with regard to tear film physiology and tear outflow. Moreover, the results demonstrate the existence of so far not recognized qualitative differences in the secretion product of main lacrimal gland and accessory lacrimal glands (glands of Krause).

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References

  • Argüeso P, Spurr-Michaud S, Russo CL, Tisdale A, Gipson IK (2003) MUC16 mucin is expressed by the human ocular surface epithelia and carries the H185 carbohydrate epitope. Invest Ophthalmol Vis Sci 44:2487–2495

    Article  PubMed  Google Scholar 

  • Argüeso P, Tisdale A, Spurr-Michaud S, Sumiyoshi M, Gipson IK (2006a) Mucin characteristics of human corneal-limbal epithelial cells that exclude the rose bengal anionic dye. Invest Ophthalmol Vis Sci 47:113–119

    Article  Google Scholar 

  • Argüeso P SM, Tisdale A, Gipson IK (2006b) Mucin O-glycan prevent apical cell-cell adhesion in corneal epithelial cells under dynamic flow conditions. ARVO Abstract 5430

  • Auersperg N, Pan J, Grove BD, Peterson T, Fisher J, Maines-Bandiera S, Somasiri A, Roskelley CD (1999) E-cadherin induces mesenchymal-to-epithelial transition in human ovarian surface epithelium. Proc Natl Acad Sci USA 96:6249–6254

    Article  PubMed  CAS  Google Scholar 

  • Bast RC, Jr., Feeney M, Lazarus H, Nadler LM, Colvin RB, Knapp RC (1981) Reactivity of a monoclonal antibody with human ovarian carcinoma. J Clin Invest 68:1331–1337

    Article  PubMed  Google Scholar 

  • Carraway KL, Ramsauer VP, Haq B, Carothers Carraway CA (2003) Cell signaling through membrane mucins. Bioessays 25:66–71

    Article  PubMed  CAS  Google Scholar 

  • Danjo Y, Watanabe H, Tisdale AS, George M, Tsumura T, Abelson MB, Gipson IK (1998) Alteration of mucin in human conjunctival epithelia in dry eye. Invest Ophthalmol Vis Sci 39:2602–2609

    PubMed  CAS  Google Scholar 

  • Gendler SJ (2001) MUC1, the renaissance molecule. J Mammary Gland Biol Neoplasia 6:339–353

    Article  PubMed  CAS  Google Scholar 

  • Higuchi T, Orita T, Nakanishi S, Katsuya K, Watanabe H, Yamasaki Y, Waga I, Nanayama T, Yamamoto Y, Munger W, Sun HW, Falk RJ, Jennette JC, Alcorta DA, Li H, Yamamoto T, Saito Y, Nakamura M (2004) Molecular cloning, genomic structure, and expression analysis of MUC20, a novel mucin protein, up-regulated in injured kidney. J Biol Chem 279:1968–1979

    Article  PubMed  CAS  Google Scholar 

  • Hori Y, Spurr-Michaud S, Russo CL, Argueso P, Gipson IK (2004) Differential regulation of membrane-associated mucins in the human ocular surface epithelium. Invest Ophthalmol Vis Sci 45:114–122

    Article  PubMed  Google Scholar 

  • Hori Y, Spurr-Michaud SJ, Russo CL, Argueso P, Gipson IK (2005) Effect of retinoic acid on gene expression in human conjunctival epithelium: secretory phospholipase A2 mediates retinoic acid induction of MUC16. Invest Ophthalmol Vis Sci 46:4050–4061

    Article  PubMed  Google Scholar 

  • Hori Y, Argueso P, Spurr-Michaud S, Gipson IK (2006) Mucins and contact lens wear. Cornea 25:176–181

    Article  PubMed  Google Scholar 

  • Jumblatt JE, Cunningham LT, Li Y, Jumblatt MM (2002) Characterization of human ocular mucin secretion mediated by 15(S)-HETE. Cornea 21:818–824

    Article  PubMed  Google Scholar 

  • Laurie GW WN, Raab RW, McKown RL (2006) NFAT/mTOR signaling and downstream promotion of MUC16 expression by lacrimal prosecretory mitogen lacritin. ARVO Abstract 1606

  • Ligtenberg MJ, Kruijshaar L, Buijs F, van Meijer M, Litvinov SV, Hilkens J (1992) Cell-associated episialin is a complex containing two proteins derived from a common precursor. J Biol Chem 267:6171–6177

    PubMed  CAS  Google Scholar 

  • Lomako J, Lomako WM, Decker SJ, Carraway CA, Carraway KL (2005) Non-apoptotic desquamation of cells from corneal epithelium: putative role for Muc4/sialomucin complex in cell release and survival. J Cell Physiol 202:115–124

    Article  PubMed  CAS  Google Scholar 

  • Lomako J, Lomako WM, Carraway CA, Carraway KL (2006) Post-translation regulation of Muc4 in cultured corneal epithelium cells by proteolytic degradation. ARVO Abstract 1608

  • McLemore MR, Aouizerat B (2005) Introducing the MUC16 gene: implications for prevention and early detection in epithelial ovarian cancer. Biol Res Nurs 6:262–267

    Article  PubMed  Google Scholar 

  • McNamara NA, Andika R, Kwong M, Sack RA, Fleiszig SM (2005) Interaction of Pseudomonas aeruginosa with human tear fluid components. Curr Eye Res 30:517–525

    Article  PubMed  CAS  Google Scholar 

  • Moniaux N, Escande F, Porchet N, Laine A, Aubert JP (2001) Structural organization and classification of the human mucin genes. Front Biosci 6:D1192–1206

    PubMed  CAS  Google Scholar 

  • Paulsen F (2006) Cell and molecular biology of human lacrimal gland and nasolacrimal duct mucins. Int Rev Cytol 249:229–279

    PubMed  CAS  Google Scholar 

  • Paulsen F, Berry M (2006) Mucins and TFF peptides of the tear film and lacrimal apparatus. Prog Histochem Cytochem 41:1–56

    Article  PubMed  CAS  Google Scholar 

  • Paulsen F, Langer G, Hoffmann W, Berry M (2004) Human lacrimal gland mucins. Cell Tissue Res 316:167–177

    Article  PubMed  CAS  Google Scholar 

  • Rossi EA, McNeer RR, Price-Schiavi SA, Van den Brande JM, Komatsu M, Thompson JF, Carraway CA, Freqien NL, Carraway KL (1996) Sialomucin complex, a heterodimeric glycoprotein complex. Expression as a soluble, secretable form in lactating mammary gland and colon. J Biol Chem 271:33476–33485

    Article  PubMed  CAS  Google Scholar 

  • Sheng ZQ, Hull SR, Carraway KL (1990) Biosynthesis of the cell surface sialomucin complex of ascites 13762 rat mammary adenocarcinoma cells from a high molecular weight precursor. J Biol Chem 265:8505–8510

    PubMed  CAS  Google Scholar 

  • Varoga D, Pufe T, Harder J, Meyer-Hoffert U, Mentlein R, Schröder JM, Petersen WJ, Tillmann BN, Proksch E, Goldring MB, Paulsen FP (2004) Production of endogenous antibiotics in articular cartilage. Arthritis Rheum 50:3526–3534

    Article  PubMed  CAS  Google Scholar 

  • Yin BW, Lloyd KO (2001) Molecular cloning of the CA125 ovarian cancer antigen: identification as a new mucin, MUC16. J Biol Chem 276:27371–27375

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

The authors thank Ute Beyer and Michaela Risch for expert technical assistance as well as Monica Berry, Academic Unit of Ophthalmology, University of Bristol, UK for her critical suggestions. Supported in part by the Deutsche Forschungs-gemeinschaft (DFG)—program grants PA 738/9-1 and PA 738/1-5, the BMBF—Wilhelm Roux Program, Halle, Germany—program grants FKZ 9/18, 12/08, and 13/08, as well as Sicca Forschungsförderung of Association of German Ophthalmologists.

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Authors and Affiliations

  1. Department of Anatomy and Cell Biology, Martin-Luther-University Halle-Wittenberg, Grosse Steinstr. 52, 06097, Halle, Saale, Germany

    Kristin Jäger, Guangxi Wu, Fabian Garreis, Lars Bräuer & Friedrich P. Paulsen

  2. School of Life Science, Fudan University, Shanghai, People’s Republic of China

    Guangxi Wu

  3. Department of Ophthalmology, Martin-Luther-University Halle-Wittenberg, Halle, Saale, Germany

    Saadettin Sel

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  1. Kristin Jäger
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  2. Guangxi Wu
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  3. Saadettin Sel
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  4. Fabian Garreis
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  5. Lars Bräuer
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  6. Friedrich P. Paulsen
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Correspondence to Friedrich P. Paulsen.

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Jäger, K., Wu, G., Sel, S. et al. MUC16 in the lacrimal apparatus. Histochem Cell Biol 127, 433–438 (2007). https://doi.org/10.1007/s00418-006-0246-6

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  • DOI: https://doi.org/10.1007/s00418-006-0246-6

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