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Ultrasonic Doppler measurements of blood flow velocity of rabbit retinal vessels using a 45-MHz needle transducer

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Abstract

Background

The purpose of this study is to measure blood flow velocity of rabbit retinal vessels using a 45-MHz ultrasonic Doppler system with a needle transducer.

Methods

A high-frequency pulsed Doppler system that utilizes a 45-MHz PMN-PT needle transducer was developed to measure retinal blood flow velocity in situ. The pulsed Doppler allowed the differentiation of retinal from choroidal blood flow velocity. The needle transducer was inserted into the vitreous cavity through a 20-gauge incision port to access the retinal vessels. The first phase of the experiment evaluated the reproducibility of the measurements. The second phase measured velocities at four positions from the optic disc edge to the distal part of each vessel in nine eyes for the temporal and six eyes for the nasal portions. The angle between the transducer and the retinal vessel at each site was measured in enucleated rabbit eyes to estimate and compensate for measurement errors.

Results

In the first phase, the average measurement error was 5.97 ± 1.34%. There was no significant difference comparing all eyes. In the second phase, the velocities gradually slowed from the disc edge to the distal part, and temporal velocities were faster than nasal velocities at all measurement sites.

Conclusion

This study demonstrated the feasibility of reliably measuring retinal blood flow velocity using a 45-MHz ultrasonic Doppler system with a needle transducer.

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Authors and Affiliations

  1. Doheny Retina Institute, Los Angeles, CA, USA

    Naoki Matsuoka, Hossein Ameri, Walid Abdallah, Amani Fawzi & Mark Humayun

  2. Doheny Eye Institute, 1450 San Pablo Street DVRC 119, Los Angeles, CA, 90033, USA

    Naoki Matsuoka, Hossein Ameri, Walid Abdallah, Amani Fawzi & Mark Humayun

  3. Division of Ophthalmology and Visual Science, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Japan

    Naoki Matsuoka

  4. Marine Industrial Engineering, Cheju National University, Jeju, South Korea

    Dong-Guk Paeng

  5. Department of Biomedical Engineering, University of Southern California, Los Angeles, CA, USA

    Ruimin Chen, Qifa Zhou & K. K. Shung

  6. Cedars-Sinai Medical Center, Los Angeles, CA, USA

    Hossein Ameri

  7. Department of Ophthalmology, Keck School of Medicine of the University of Southern California, Los Angeles, CA, USA

    Amani Fawzi & Mark Humayun

Authors
  1. Naoki Matsuoka
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  2. Dong-Guk Paeng
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  3. Ruimin Chen
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  4. Hossein Ameri
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  5. Walid Abdallah
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  6. Qifa Zhou
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  7. Amani Fawzi
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  8. K. K. Shung
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  9. Mark Humayun
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Corresponding author

Correspondence to Mark Humayun.

Additional information

Dong-Guk Paeng contributed equally to this work.

Supported in part by grants from NEI grant EY03040. An unrestricted grant form Research to Prevent Blindness and funding through private donations were also given.

The authors would like to thank Dr. Xiaochen Xu who fabricated the transducer and did basic experiments.

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Matsuoka, N., Paeng, DG., Chen, R. et al. Ultrasonic Doppler measurements of blood flow velocity of rabbit retinal vessels using a 45-MHz needle transducer. Graefes Arch Clin Exp Ophthalmol 248, 675–680 (2010). https://doi.org/10.1007/s00417-009-1298-9

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  • DOI: https://doi.org/10.1007/s00417-009-1298-9

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