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Peptide YY induces enterocyte proliferation in a rat model with total enteral nutrition after distal bowel resection

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Abstract

The main reason why enterocyte proliferative effects of peptide YY (PYY) have not been detected in rats undergoing massive small intestinal resection after feeding may have been the background activity of markedly increased endogenous PYY released from L cells in the distal gut in response to the intraluminal nutrients. The purpose of the present study was to evaluate the effects of PYY on enterocyte proliferation in a rat model of distal bowel resection (DBR) with total enteral nutrition (TEN). Male, adult Sprague–Dawley rats were assigned into three experimental groups: sham rats underwent bowel transection and reanastomosis, DBR rats underwent the resection of 40 cm distal small intestine and colon, and DBR–PYY rats underwent distal bowel resection as above, and were treated with PYY1–36 from day 2 to day 14 postoperatively. During days 2–14 postoperatively, all animals received isocaloric TEN. At the endpoints, plasma PYY levels and parameters of enterocyte proliferation were determined. Compared with the sham group, DBR rats demonstrated a significant decrease in plasma PYY levels, and a significant increase in intestinal bowel and mucosal weight, mucosal DNA and protein content, villus height and crypt depth, and crypt cell proliferation index. Administration of PYY (DBR-PYY group) led to a significant increase in plasma PYY levels, intestinal bowel and mucosal weight, mucosal DNA and protein content, villus height and crypt depth, and crypt cell proliferation index in comparison with the DBR untreated group. We conclude that administration of PYY increases the plasma PYY levels, and PYY induces enterocyte proliferation with TEN after distal bowel resection.

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References

  1. Tatemoto K, Mutt V (1980) Isolation of two novel candidate hormones using a chemical method for finding naturally occurring polypeptides. Nature 285:417–418. doi:10.1038/285417a0

    Article  PubMed  CAS  Google Scholar 

  2. Guo YS, Fujimura M, Lluis F et al (1987) Inhibitory action of peptide YY on gastric acid secretion. Am J Physiol 253:G298–302

    PubMed  CAS  Google Scholar 

  3. Nightingale JM, Kamm MA, van der Siip JR et al (1996) Gastrointestinal hormones in short bowel syndrome. Peptide YY may be the ‘colonic brake’ to gastric emptying. Gut 39:267–272. doi:10.1136/gut.39.2.267

    Article  PubMed  CAS  Google Scholar 

  4. Liu CD, Aloia T, Adrian TE et al (1996) Peptide YY: a potential proabsorptive hormone for the treatment of malabsorptive disorders. Am Surg 62:232–236

    PubMed  CAS  Google Scholar 

  5. Adrian TE, Bacarese-Hamilton AJ, Smith HA et al (1987) Distribution and postprandial release of porcine peptide YY. J Endocr 113:11–14

    PubMed  CAS  Google Scholar 

  6. Gleeley GH Jr, Hashimoto T, Izukura M et al (1989) A comparison of intraduodenally, and intracolonically administered nutrients on the release of peptide YY in the dog. Endocrinology 125:1761–1765

    Article  Google Scholar 

  7. Taylor IL (1985) Distribution and release of peptide YY in dogs measured by specific radioimmunoassay. Gastroenterology 88:731–737

    PubMed  CAS  Google Scholar 

  8. Imamura M, Takahashi H, Mikami Y et al (1998) Elevation of plasma peptide YY and pancreatic juice hypersecretion following massive small bowel resection in the rat. Tohoku J Exp Med 184:49–59. doi:10.1620/tjem.184.49

    Article  PubMed  CAS  Google Scholar 

  9. Goodlad RA, Lenton W, Ghatei MA et al (1987) Effects of an elemental diet, inert bulk and different types of dietary fibre on the response of the intestinal epithelium to refeeding in the rat and relationship to plasma gastrin, enteroglucagon, and PYY concentrations. Gut 28:171–180. doi:10.1136/gut.28.2.171

    Article  PubMed  CAS  Google Scholar 

  10. Chance WT, Zhang X, Balasubramaniam A et al (1996) Preservation of intestine protein by peptide YY during total parenteral nutrition. Life Sci 58:1785–1794. doi:10.1016/0024-3205(96)00162-2

    Article  PubMed  CAS  Google Scholar 

  11. Savage AP, Gornacz GE, Adrian TE et al (1985) Is raised plasma peptide YY after intestinal resection in the rat responsible for the trophic response? Gut 26:1353–1358. doi:10.1136/gut.26.12.1353

    Article  PubMed  CAS  Google Scholar 

  12. Imamura M, Nakajima H, Mikami Y et al (1994) Morphological and immunohistochemical changes in intestinal mucosa and PYY release following total colectomy with ileal pouch–anal anastomosis in dogs. Dig Dis Sci 44:1000–1007. doi:10.1023/A:1026620900259

    Article  Google Scholar 

  13. Gomez G, Zhang T, Rajaraman S et al (1995) Intestinal peptide YY: ontogeny of gene expression in rat bowel and trophic actions on rat and mouse bowel. Am J Physiol 268:G71–81

    PubMed  CAS  Google Scholar 

  14. Fukatsu K, Moriya T, Maeshima Y et al (2005) Exogenous interleukin 7 affects gut-associated lymphoid tissue in mice receiving total parenteral nutrition. Shock 24:541–546. doi:10.1097/01.shk.0000183237.32256.78

    Article  PubMed  CAS  Google Scholar 

  15. Dowling RH, Booth CC (1967) Structural and functional changes following small intestinal resection in the rats. Clin Sci 32:139–149

    PubMed  CAS  Google Scholar 

  16. Chomszynski P (1993) A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples. Biotechniques 15:532–536

    Google Scholar 

  17. Hazelwood RL (1993) The pancreatic polypeptide (PP-fold) family: gastrointestinal, vascular, and feeding behavioral implications. Proc Soc Exp Biol Med 202:44–63

    PubMed  CAS  Google Scholar 

  18. Wilander E, El-Salhy M, Lundqvist M et al (1983) Polypeptide YY (PYY), and pancreatic polypeptide (PP) in rectal carcinoids an immunocytochemical study. Virchows Arch A Pathol Anat Histopathol 401:67–72. doi:10.1007/BF00644790

    Article  PubMed  CAS  Google Scholar 

  19. Adiran TE, Ferri GL, Bacarese-Hamilton AJ et al (1985) Human distribution and release of a putative new gut hormone, peptide YY. Gastroenterology 89:1070–1077

    Google Scholar 

  20. Booth IW, Lander AD (1998) Short bowel syndrome. Baillieres Clin Gastroenterol 12:739–772. doi:10.1016/S0950-3528(98)90006-9

    Article  PubMed  CAS  Google Scholar 

  21. Burrington JD (1971) Surgery after massive small bowel resection. Am J Surg 121:213–214. doi:10.1016/0002-9610(71)90190-5

    Article  PubMed  CAS  Google Scholar 

  22. Pereira PM, Bines JE (2006) New growth factor therapies aimed at improving intestinal adaptation in short bowel syndrome. J Gastroenterol Hepatol 21:932–940. doi:10.1111/j.1440-1746.2006.04351.x

    Article  PubMed  Google Scholar 

  23. O’ Brien DP, Nelson LA, Huang FS et al (2001) Intestinal adaptation: structure, function, and regulation. Semin Pediatr Surg 10:56–64. doi:10.1053/spsu.2001.22383

    Article  CAS  Google Scholar 

  24. Mohr AJ, Leisewitz AL, Jacobson LS et al (2003) Effect of early enteral nutrition on intestinal permeability, intestinal protein loss, and outcome in dogs with severe parvoviral enteritis. J Vet Intern Med 17:791–798. doi:10.1892/0891-6640(2003)017<0791:EOEENO>2.3.CO;2

    Article  PubMed  Google Scholar 

  25. Jeejeehoy KN (2007) Enteral nutrition versus parenteral nutrition—the risks and benefits. Nat Clin Pract Gastroenterol Hepatol 4:260–265. doi:10.1038/ncpgasthep0797

    Article  Google Scholar 

  26. Adrian TE, Savage AP, Fuessl HS et al (1987) Release of peptide YY (PYY) after resection of small bowel, colon, or pancreas in man. Surgery 101:715–719

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Research Institute of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing, China

    Wei Zhang, Ning Li, Weiming Zhu, Yichao Shi, Jian Zhang, Qiurong Li & Jieshou Li

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  1. Wei Zhang
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  2. Ning Li
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  3. Weiming Zhu
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  4. Yichao Shi
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  5. Jian Zhang
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  6. Qiurong Li
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  7. Jieshou Li
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Correspondence to Weiming Zhu.

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Zhang, W., Li, N., Zhu, W. et al. Peptide YY induces enterocyte proliferation in a rat model with total enteral nutrition after distal bowel resection. Pediatr Surg Int 24, 913–919 (2008). https://doi.org/10.1007/s00383-008-2176-y

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  • DOI: https://doi.org/10.1007/s00383-008-2176-y

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