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Ammonia transport across the skin of adult rainbow trout (Oncorhynchus mykiss) exposed to high environmental ammonia (HEA)

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Abstract

Recent molecular evidence points towards a capacity for ammonia transport across the skin of adult rainbow trout. A series of in vivo and in vitro experiments were conducted to understand the role of cutaneous ammonia excretion (J amm) under control conditions and after 12-h pre-exposure to high environmental ammonia (HEA; 2 mmol/l NH4HCO3). Divided chamber experiments with bladder-catheterized, rectally ligated fish under light anesthesia were performed to separate cutaneous J amm from branchial, renal, and intestinal J amm. Under control conditions, cutaneous J amm accounted for 4.5 % of total J amm in vivo. In fish pre-exposed to HEA, plasma total ammonia concentration increased 20-fold to approximately 1,000 μmol/l, branchial J amm increased 1.5- to 2.7-fold, and urinary J amm increased about 7-fold. Urinary J amm still accounted for less than 2 % of total J amm. Cutaneous J amm increased 4-fold yet amounted to only 5.7 % of total J amm in these fish. Genes (Rhcg1, Rhcg2, Rhbg, NHE-2, v-type H+-ATPase) known to be involved in ammonia excretion at the gills of trout were all expressed at the mRNA level in the skin, but their expression did not increase with HEA pre-exposure. In vitro analyses using [14C] methylamine (MA), an ammonia analog which is transported by Rh proteins, demonstrated that MA permeability in isolated skin sections was higher in HEA pre-exposed fish than in control fish. The addition of basolateral ammonia (1,000 μmol/l) to this system abolished this increase in permeability, suggesting ammonia competition with MA for Rh-mediated transport across the skin of HEA pre-exposed trout; this did not occur in skin sections from control trout. Moreover, in vitro J amm by the skin of fish which had been pre-exposed to HEA was also higher than in control fish in the absence of basolateral ammonia, pointing towards a possible cutaneous ammonia loading in response to HEA. In vitro MA permeability was reduced upon the addition of amiloride (10−4 mol/l), but not phenamil (10−5 mol/l) suggesting a role for a Na/H-exchanger (NHE) in cutaneous ammonia transport, as has been previously described in the skin of larval fish. Overall, it appears that under control conditions and in response to HEA pre-exposure, the skin makes only a very minor contribution to total J amm, but the observed increases in cutaneous J amm in vivo and in cutaneous J amm and MA permeability in vitro demonstrate the capacity for ammonia transport in the skin of adult trout. It remains unclear if this capacity may become significant under certain environmental challenges or if it is merely a remnant of cutaneous transport capacity from early life stages in these fish.

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Acknowledgments

Special thanks to Ryan Belowitz for assistance in skin surface area measurements and analyses, and to Jeff Richards and Eric Clelland for arranging access to [14C]methylamine. This research was supported by NSERC (Natural Sciences and Engineering Research Council of Canada) Discovery Grants to CJB and CMW, who is also supported by the Canada Research Chair Program. AZ was supported by an Ontario Graduate Scholarship.

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  1. Department of Biology, McMaster University, Hamilton, ON, L8S 4K1, Canada

    Alex M. Zimmer & Chris M. Wood

  2. Department of Zoology, University of British Columbia, Vancouver, BC, V6T 1Z4, Canada

    Alex M. Zimmer, Colin J. Brauner & Chris M. Wood

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  1. Alex M. Zimmer
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Correspondence to Alex M. Zimmer.

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Communicated by G. Heldmaier.

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Zimmer, A.M., Brauner, C.J. & Wood, C.M. Ammonia transport across the skin of adult rainbow trout (Oncorhynchus mykiss) exposed to high environmental ammonia (HEA). J Comp Physiol B 184, 77–90 (2014). https://doi.org/10.1007/s00360-013-0784-x

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  • DOI: https://doi.org/10.1007/s00360-013-0784-x

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