Abstract.
We demonstrate third-harmonic microscopy of thin biological objects using a Ti:sapphire laser source. A very simple modification to the collection and detection systems of the microscope allows for the detection of the third-harmonic signal near 270 nm. We show that, using a Ti:sapphire laser, we can combine third-harmonic imaging with two-photon excitation fluorescence simultaneously. Compared to other possible laser sources for third-harmonic microscopy, the Ti:sapphire laser provides better optical resolution, better power availability, and is less absorbed in water. We find that the Ti:sapphire laser is the most suitable laser source for third-harmonic microscopy for thin biological specimens.
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Received: 16 September 2001 / Revised version: 29 October 2001 / Published online: 27 June 2002
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Yelin, D., Oron, D., Korkotian, E. et al. Third-harmonic microscopy with a titanium–sapphire laser. Appl Phys B 74 (Suppl 1), s97–s101 (2002). https://doi.org/10.1007/s00340-002-0884-x
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DOI: https://doi.org/10.1007/s00340-002-0884-x