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Inheritance in doubled-diploid clementine and comparative study with SDR unreduced gametes of diploid clementine

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Abstract

Key message

Tetraploid clementine displays mainly tetrasomic inheritance. Genetic structures of 2n SDR and 2 × gametes from DD clementine are complementary and will guides triploids citrus breeding strategies.

Abstract

Triploid breeding is developed worldwide to create new seedless cultivars. Citrus triploid hybrids can be recovered from 2x × 2x sexual hybridizations as a consequence of the formation of unreduced gametes (2n), or from 4x × 2x interploid hybridizations in which tetraploid parents used are most often doubled-diploid (DD). Here we have analyzed the inheritance in doubled-diploid clementine and compared the genetic structures of gametes of DD clementine with SDR unreduced gametes of diploid clementine. Parental heterozygosity restitution (PHR) with DD parents depends on the rate of preferential chromosome pairing and thus the proportion of disomic versus tetrasomic segregations. Doubled-diploid clementine largely exhibited tetrasomic segregation. However, three linkage groups had intermediate segregation and one had a tendency for disomy. Significant doubled reduction rates (DR) rates were observed in six of the nine LGs. Differences of PHR between 2n SDR and 2x DD gametes were highest in the centromeric region and progressively decreased toward the distal regions where they were not significant. Over all markers, PHR was lower (two-thirds) in SDR 2n gametes than in DD-derived diploid gametes. The two strategies appear complementary in terms of genotypic variability. Interploid 4x × 2x hybridization is potentially more efficient for developing new cultivars that are phenotypically closer to the diploid parent of the DD than sexual hybridization through SDR 2n gametes. Conversely, 2x × 2x triploidisation has the potential to produce novel products with characteristics for market segmentation strategies.

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Abbreviations

CIs:

Confidence Intervals

DD:

Doubled-Diploid

DR:

Double Reduction rate

FDR:

First Division Restitution

FRET:

Förster Resonance Energy Transfer

LG:

Linkage Group

LOD:

Log-odds ratio

MAC-PR:

Microsatellite DNA Allele Counting-Peak Ratio

PHR:

Parental Heterozygosity Restitution

PPP:

Referential Pairing

SDR:

Second-Division Restitution

SNP:

Single Nucleotide Polymorphism

SSR:

Simple Sequence Repeat

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Acknowledgments

This work was supported by Grant AGL2011-26490, from the Ministry of ‘Economía y Competividad’, Fondo Europeo de Desarrollo Regional (FEDER).

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Authors and Affiliations

  1. Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera km 4.5, 46113, Moncada, Valencia, Spain

    P. Aleza, J. Cuenca, J. Juárez, L. Navarro & P. Ollitrault

  2. UMR AGAP, Centre de coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), Station de Roujol, 97170, Petit-Bourg, Guadeloupe

    P. Ollitrault

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  1. P. Aleza
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  2. J. Cuenca
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Correspondence to L. Navarro or P. Ollitrault.

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Aleza, P., Cuenca, J., Juárez, J. et al. Inheritance in doubled-diploid clementine and comparative study with SDR unreduced gametes of diploid clementine. Plant Cell Rep 35, 1573–1586 (2016). https://doi.org/10.1007/s00299-016-1972-4

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