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Phenotypic and molecular evaluation of cotton hairy roots as a model system for studying nematode resistance

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Abstract

Agrobacterium rhizogenes-induced cotton (Gossypium hirsutum L.) hairy roots were evaluated as a model system for studying molecular cotton–nematode interactions. Hairy root cultures were developed from the root-knot nematode (RKN) (Meloidogyne incognita [Kofoid and White] Chitwood, race 3)-resistant breeding line M315 and from the reniform nematode (RN) (Rotylenchulus reniformis Linford & Oliveira)-resistant accession GB713 (G. barbadense L.) and compared to a nematode-susceptible culture derived from the obsolete cultivar DPL90. M315, GB713, and DPL90 hairy roots differed significantly in their appearance and growth potential; however, these differences were not correlated with transcript levels of the A. rhizogenes T-DNA genes rolB and aux2 which help regulate hairy root initiation and proliferation. DPL90 hairy roots were found to support both RKN and RN reproduction in tissue culture, whereas M315 and GB713 hairy roots were resistant to RKN and RN, respectively. M315 hairy roots showed constitutive up-regulation of the defense gene MIC3 (Meloidogyne Induced Cotton3) compared to M315 whole-plant roots and DPL90 hairy roots. Our data show the potential use of cotton hairy roots in maintaining monoxenic RKN and RN cultures and suggest hairy roots may be useful in evaluating the effect of manipulated host gene expression on nematode resistance in cotton.

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Acknowledgments

The authors would like to thank Dr. Dirk Charlson (University of Arkansas) and Dr. Jeff Wilkinson (Mississippi State University) for their critical review of the manuscript.

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Authors and Affiliations

  1. Crop Science Research Laboratory, USDA/ARS, Mississippi State, MS, 39762, USA

    Martin J. Wubben, Franklin E. Callahan & Johnie N. Jenkins

  2. Southern Regional Research Center, USDA/ARS, New Orleans, LA, 70124, USA

    Barbara A. Triplett

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  1. Martin J. Wubben
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  2. Franklin E. Callahan
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  3. Barbara A. Triplett
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  4. Johnie N. Jenkins
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Correspondence to Martin J. Wubben.

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Communicated by J. Register.

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Wubben, M.J., Callahan, F.E., Triplett, B.A. et al. Phenotypic and molecular evaluation of cotton hairy roots as a model system for studying nematode resistance. Plant Cell Rep 28, 1399–1409 (2009). https://doi.org/10.1007/s00299-009-0739-6

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  • DOI: https://doi.org/10.1007/s00299-009-0739-6

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