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A novel anti-calcification method for bioprosthetic heart valves using dopamine-modified alginate

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Abstract

Calcification is one of the main causes for bioprosthetic heart valves (BHVs)’ failure. Reported strategies to improve BHVs’ anti-calcification properties only target some of risk factors for calcification. In the current study, we demonstrated dopamine-modified alginate coating served as a protected layer for BHVs’ anti-calcification. Alginate coating was characterized by infrared spectroscopic analysis, ultraviolet spectrophotometer, and water contact angle test. By both high-calcium and high-phosphorus in vitro calcification model and rat subdermal implant in vivo calcification model, our results showed alginate-coated BHVs’ have greatly improved BHVs’ anti-calcification performance due to constant ionic exchange of calcium and sodium. Besides, alginate-coated BHVs did not change BHVs’ mechanical strength and tissue’s shrink temperature. This was the first proof-of-concept study to verify that alginate coating would be a novel method for BHVs’ anti-calcification.

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Abbreviations

BHVs:

Bioprosthetic heart valves

EDC:

1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide

Dop–Alg:

Dopamine-modified alginate

GAGs:

Glycosaminoglycans

GLUT:

Glutaraldehyde

NHS:

N-hydroxysuccinimide

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Acknowledgements

This work is supported by National Natural Science Foundation of China (31700833), Young Elite Scientists Sponsorship Program by CAST (2017QNRC001), the Fundamental Research Funds for the Central Universities (YJ201641), National Key Research and Development Programs (2017YFC1104200, 2016YFC1102200), and the Program of Introducing Talents of Discipline to Universities (111 Project, No. B16033).

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Authors and Affiliations

  1. National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, 610064, China

    Yanfei Hu, Xin Su, Yang Lei & Yunbing Wang

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  1. Yanfei Hu
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  2. Xin Su
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Correspondence to Yang Lei or Yunbing Wang.

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Fig. S1

A. UV spectrophotometer for dopamine. B. The optimization of concentration for Dop-Alg solution. N = 3(three independent experiments) (PPTX 69 kb)

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Hu, Y., Su, X., Lei, Y. et al. A novel anti-calcification method for bioprosthetic heart valves using dopamine-modified alginate. Polym. Bull. 76, 1423–1434 (2019). https://doi.org/10.1007/s00289-018-2450-7

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