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Viscera-Associated Bacterial Diversity Among Intertidal Gastropods from Northern-Atlantic Coast of Portugal

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Abstract

Culture-dependent evaluation of the bacteria was carried out on gastropods, such as Monodonta lineata, Gibbula umbilicalis, Nucella lapillus and Patella intermedia, and the environmental samples (biofilm and surrounding sea water) collected from six different locations of Northern Portugal coastal area to investigate the interactions between the microbes in the viscera of gastropods and in the environment. A total of 141 isolates and 39 operational taxonomic units were identified. Phylogenetic analysis based on the 16S rRNA gene showed that bacterial isolates are highly diverse and most of them were found in other marine environment. The observed bacterial diversity was distributed over five different classes (Gammaproteobacteria, Alphaproteobacteria, Flavobacteria, Bacilli and Actinobacteria) with the greatest number of 16S rRNA gene sequence derived from the Gammaproteobacteria (77 %). Vibrio is found to be the dominant one among the different bacterial species isolated. The results suggest that the microorganisms in the environment are maintained in the viscera of the gastropods which may have a key role in the metabolic functions.

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Acknowledgments

One of the authors V. Pratheepa thanks to the scholar Anoop Alex for helping in Phylogentic analysis and valuable suggestions in preparing the manuscript. The author is also grateful to Fundaçao para a Ciencia e Technologia (FCT), Portugal for a Postdoctoral Grant (SFRH/BPD/78269/2011) and the projects PesT-C/MAR/LA0015/2011 from the Portuguese Foundation for Science and Technology (FCT), INTERREG IV projects Atlantox and Pharmatlantic and MARBIOTECH-NORTE-07-0124-FEDER-000047.

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Authors and Affiliations

  1. CIMAR/CIIMAR, Centro Interdisciplinar de Investigação Marinha e Ambiental, Universidade do Porto, Rua dos Bragas 289, 4050-123, Porto, Portugal

    Vijaya K. Pratheepa, Marisa Silva & Vitor Vasconcelos

  2. Departamento de Biologia, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre, 4169-007, Porto, Portugal

    Marisa Silva & Vitor Vasconcelos

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  1. Vijaya K. Pratheepa
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  2. Marisa Silva
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  3. Vitor Vasconcelos
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Correspondence to Vitor Vasconcelos.

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Pratheepa, V.K., Silva, M. & Vasconcelos, V. Viscera-Associated Bacterial Diversity Among Intertidal Gastropods from Northern-Atlantic Coast of Portugal. Curr Microbiol 68, 140–148 (2014). https://doi.org/10.1007/s00284-013-0450-2

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  • DOI: https://doi.org/10.1007/s00284-013-0450-2

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