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GidA Expression in Salmonella is Modulated Under Certain Environmental Conditions

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Abstract

Glucose-inhibited division (GidA) protein is widely distributed in nature, and is highly conserved among bacteria and eukarya. In our previous study, a gidA mutant was attenuated in both in vitro and in vivo models of Salmonella infection. Furthermore, deletion of gidA resulted in a marked reduction in the expression of many virulence genes and proteins, suggesting a role for GidA in the regulation of Salmonella virulence. In this study, the effect of different environmental conditions (glucose, EDTA, and pH 5) on GidA expression in Salmonella was examined. Transcriptional analysis using real-time RT-PCR and a β-galactosidase assay, displayed no differences in gidA transcription and promoter activity in different environmental conditions. Conversely, semiquantitative Western blot analysis revealed a significant increase in the GidA expression in Salmonella when grown under different environmental conditions. Salmonella in vitro virulence assays showed an increased virulence potential in the environmental conditions correlating to the increase in GidA expression. Together, our data indicate that GidA expression is modulated under different environmental conditions which correlate to increased Salmonella in vitro virulence.

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Acknowledgments

The authors would like to express their gratitude to Dr. Richard Gourse, the University of Wisconsin-Madison, for providing the E. coli RLG3499 strain, as well as acknowledge the assistance provided during the densitometry analysis. This study was supported by a Grant from the USDA Hatch Fund.

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Authors and Affiliations

  1. Department of Animal Sciences, University of Wisconsin-Madison, 1675 Observatory Drive, Madison, WI, 53706, USA

    Jaclyn M. Rehl, Daniel C. Shippy, Nicholas M. Eakley, Megan D. Brevik, Jordan M. Sand, Mark E. Cook & Amin A. Fadl

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  1. Jaclyn M. Rehl
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  2. Daniel C. Shippy
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  3. Nicholas M. Eakley
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  4. Megan D. Brevik
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  7. Amin A. Fadl
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Correspondence to Amin A. Fadl.

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Jaclyn M. Rehl and Daniel C. Shippy contributed equally to this study.

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Rehl, J.M., Shippy, D.C., Eakley, N.M. et al. GidA Expression in Salmonella is Modulated Under Certain Environmental Conditions. Curr Microbiol 67, 279–285 (2013). https://doi.org/10.1007/s00284-013-0361-2

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  • DOI: https://doi.org/10.1007/s00284-013-0361-2

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