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Isolation of Three Xylanase-Producing Strains of Actinomycetes and Their Identification Using Molecular Methods

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Abstract

Eighty-eight actinomycetes were isolated from 20 samples collected from different locations in and around Delhi, India. Among these, 69 isolates were found positive for xylanase production. Of 69 isolates, 3 (SN32, SN77, and SN83) produced >125 IU/ml xylanase. Modern genetic tools were used for revealing the identities of these potent xylanase producers. The selected isolates were categorized under the genus Streptomyces based on their cultural and morphologic characteristics. Genetic diversity among these species of Streptomyces was established based on restriction length fragment polymorphism and random amplified polymorphic DNA analysis. The closest phylogenetic neighbours according to the 16S rRNA gene-sequence data for the three isolates SN32, SN77, and SN83 were Streptomyces cyaneus, S. tendae, and S. caelestis, respectively.

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Acknowledgments

The corresponding investigator thankfully acknowledges the study leave granted by Department of Biotechnology, Ministry of Science and Technology, Government of India, New Delhi to undertake this work.

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Authors and Affiliations

  1. Department of Biotechnology, Ministry of Science and Technology, Block II, CGO Complex, Lodhi Road, New Delhi, 110 003, India

    Suchita Ninawe

  2. Department of Zoology, University of Delhi, Delhi, 110007, India

    Rup Lal

  3. Department of Microbiology, University of Delhi South Campus, Benito Juarez Rd., New Delhi, 110 021, India

    R. C. Kuhad

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  1. Suchita Ninawe
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  2. Rup Lal
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  3. R. C. Kuhad
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Correspondence to Suchita Ninawe.

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Ninawe, S., Lal, R. & Kuhad, R.C. Isolation of Three Xylanase-Producing Strains of Actinomycetes and Their Identification Using Molecular Methods. Curr Microbiol 53, 178–182 (2006). https://doi.org/10.1007/s00284-005-0285-6

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  • DOI: https://doi.org/10.1007/s00284-005-0285-6

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