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The response of archaeal species to seasonal variables in a subtropical aerated soil: insight into the low abundant methanogens

  • Environmental biotechnology
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Abstract

Archaea are cosmopolitan in aerated soils around the world. While the dominance of Thaumarchaeota has been reported in most soils, the methanogens are recently found to be ubiquitous but with low abundances in the aerated soil globally. However, the seasonal changes of Archaea community in the aerated soils are still in the mist. In this study, we investigated the change of Archaea in the context of environmental variables over a period of 12 months in a subtropical soil on the Chongming Island, China. The results showed that Nitrososphaera spp. were the dominant archaeal population while the methanogens were in low proportions but highly diverse (including five genera: Methanobacterium, Methanocella, Methanosaeta, Methanosarcina, and Methanomassiliicoccus) in the aerated soil samples determined by high throughput sequencing. A total of 126 LSA correlations were found in the dataset including all the 72 archaeal OTUs and 8 environmental factors. A significance index defined as the pagerank score of each OTU divided by its relative abundance was used to evaluate the significance of each OTU. The results showed that five out of 17 methanogen OTUs were significantly positively correlated with temperature, suggesting those methanogens might increase with temperature rather than being dormant in the aerated soils. Given the metabolic response of methanogens to temperature under aerated soil conditions, their contribution to the global methane cycle warrants evaluation.

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Acknowledgements

We thank Yijie Wang for helping with the field work. This work was supported by The National Science Foundation for Young Scholars of China 41306123 (to WX), National Natural Science Foundation of China 31200986 (to RZ), 41530105 CZ, Natural Science Foundation, the Shanghai Committee of Science and Technology 16ZR1449800 (to RZ) and 13JC1405200 (to CZ), and the Fundamental Research Funds for the Central Universities 1350219165 (to CZ), 10247201546 and 2000219083 (to RZ). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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  1. Wei Xie and Na Jiao contributed equally.

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  1. State Key Lab of Marine Geology, Tongji University, Shanghai, 200092, People’s Republic of China

    Wei Xie, Cenling Ma & Chuanlun Zhang

  2. Department of Bioinformatics, School of Life Sciences and Technology, Tongji University, Shanghai, 200092, People’s Republic of China

    Na Jiao, Sa Fang & Ruixin Zhu

  3. Department of Earth and Planetary Sciences, University of Tennessee, Knoxville, TN, 37996, USA

    Tommy J. Phelps

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  1. Wei Xie
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Xie, W., Jiao, N., Ma, C. et al. The response of archaeal species to seasonal variables in a subtropical aerated soil: insight into the low abundant methanogens. Appl Microbiol Biotechnol 101, 6505–6515 (2017). https://doi.org/10.1007/s00253-017-8349-7

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