Abstract
Microbial oxidation potentials of extremophiles recovered from Pampo Sul oil field, Campos Basin, Brazil, in pure culture or in consortia, were investigated using high-throughput screening (HTS) and multibioreactions. Camphor (1), cis-jasmone (2), 2-methyl-cyclohexanone (3), 1,2-epoxyoctane (4), phenylethyl acetate (5), phenylethyl propionate (6), and phenylethyl octanoate (7) were used to perform multibioreaction assays. Eighty-two bacterial isolates were recovered from oil and formation water samples and those presenting outstanding activities in HTS assays were identified by sequencing their 16S rRNA genes. These results revealed that most microorganisms belonged to the genus Bacillus and presented alcohol dehydrogenase, monooxygenase, epoxide hydrolase, esterase, and lipase activities.
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Acknowledgments
This work was part of the multidisciplinary project BIOPETRO supported by FINEP and PETROBRAS. GF da Cruz and SP Vasconcellos were supported by grants from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). CFF Angolini, PF Lopes, and E. Crespim were supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Brazil), while LG Oliveira received a fellowship from Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP). We thank PETROBRAS for the authorization to publish this work. We also thank Prof. Carol Collins for helpful suggestions in style and grammar.
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da Cruz, G.F., Angolini, C.F.F., de Oliveira, L.G. et al. Searching for monooxygenases and hydrolases in bacteria from an extreme environment. Appl Microbiol Biotechnol 87, 319–329 (2010). https://doi.org/10.1007/s00253-010-2485-7
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DOI: https://doi.org/10.1007/s00253-010-2485-7