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The feline major histocompatibility complex is rearranged by an inversion with a breakpoint in the distal class I region

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Abstract

In order to determine the genomic organization of the major histocompatibility complex (MHC) of the domestic cat (Felis catus), DNA probes for 61 markers were designed from human MHC reference sequences and used to construct feline MHC BAC contig map spanning ARE1 in the class II region to the olfactory receptor complex in the extended class I region. Selected BAC clones were then used to identify feline-specific probes for the three regions of the mammalian MHC (class II–class III–class I) for radiation hybrid mapping and fluorescent in situ hybridization to refine the organization of the domestic cat MHC. The results not only confirmed that the p-arm of domestic cat B2 is inverted relative to human Chromosome 6, but also demonstrated that one inversion breakpoint localized to the distal segment of the MHC class I between TRIM39 and TRIM26. The inversion thus disjoined the ~2.85 Mb of MHC containing class II–class III–class I (proximal region) from the ~0.50 Mb of MHC class I/extended class I region, such that TRIM39 is adjacent to the Chromosome B2 centromere and TRIM26 is adjacent to the B2 telomere in the domestic cat.

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Acknowledgements

The authors gratefully acknowledge to the technical assistance of H. Banh, M. Clotter, N. Crumpler, C. Jones, M. Kessler, and S. Selway. In addition, the authors are indebted to Drs. Pat Martin and Colm O’hUigin for critical reading of this manuscript and R. Stephens (Advanced Biomedical Computing Center, National Cancer Institute at Frederick). This publication has been funded in whole or part with F

federal funds from the National Cancer Institute, National Institutes of Health, under contract no. N01-C0-12400. The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does mention of trade names, commercial products, or organizations imply endorsement by the US Government.

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Authors and Affiliations

  1. Basic Research Program, SAIC-Frederick, National Cancer Institute-Frederick, Frederick, MD, 21702-1201, USA

    Thomas W. Beck, Joan Menninger & William J. Murphy

  2. H&W Cytogenetics Services, Lovettsville, VA, 20180, USA,

    William G. Nash

  3. Laboratory of Genomic Diversity, National Cancer Institute-Frederick, Frederick, MD, 21702-1201, USA

    Stephen J. O’Brien & Naoya Yuhki

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  1. Thomas W. Beck
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  2. Joan Menninger
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  3. William J. Murphy
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  4. William G. Nash
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  5. Stephen J. O’Brien
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Correspondence to Thomas W. Beck or Naoya Yuhki.

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Beck, T.W., Menninger, J., Murphy, W.J. et al. The feline major histocompatibility complex is rearranged by an inversion with a breakpoint in the distal class I region. Immunogenetics 56, 702–709 (2005). https://doi.org/10.1007/s00251-004-0742-6

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  • DOI: https://doi.org/10.1007/s00251-004-0742-6

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