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Analysis of Microsatellites in 13 Hemiascomycetous Yeast Species: Mechanisms Involved in Genome Dynamics

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Abstract

We have analyzed all di-, tri-, and tetranucleotide repeats in the partially sequenced genomes of 13 hemiascomycetous yeast species, and compared their sequences, lengths, and distributions to those observed in the genome of Saccharomyces cerevisiae. We found that most of the 13 species exhibit a unique distribution of microsatellites, not correlated to the base composition of their genome. Species close to S. cerevisiae exhibit a similar distribution, while species more distantly related show a more divergent distribution. We propose that de novo formation and continuous loss of microsatellites are active processes generating new DNA sequences. We also show that hemiascomycete-specific genes encoding transcription factors contain trinucleotide repeats more frequently than expected from their average frequency distribution. These transcription factors might play an important role in the speciation process, by regulating gene expression through DNA–protein or protein–protein interactions mediated by stretches of charged amino acids encoded by trinucleotide repeats.

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Acknowledgements

We are grateful to our colleagues of the Génolevures project (http://cbi.labri.u-bordeaux.fr/Genolevures ) for giving us access to their sequence data before publication. We thank Florence Ricard, Gäelle Blandin, and Fredj Tekaia for excellent computer assistance and our colleagues of the Unité de Génétique Moléculaire des Levures for fruitful discussions. This work was supported by CNRS as part of the GDR 2354. B.D. is a member of the Institut Universitaire de France.

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  1. Unité de Génétique Moléculaire des Levures (URA 2171 CNRS, UFR 927 Université Pierre et Marie Curie), Institut Pasteur, 25 rue du Dr. Roux, 75724 Paris cedex 15, France

    Alain Malpertuy, Bernard Dujon & Guy-Franck Richard

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  1. Alain Malpertuy
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  2. Bernard Dujon
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Correspondence to Guy-Franck Richard.

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Malpertuy, A., Dujon, B. & Richard, GF. Analysis of Microsatellites in 13 Hemiascomycetous Yeast Species: Mechanisms Involved in Genome Dynamics . J Mol Evol 56, 730–741 (2003). https://doi.org/10.1007/s00239-002-2447-5

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  • DOI: https://doi.org/10.1007/s00239-002-2447-5

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